The interference of oxygen on diaphorase from Clostridium kluveri in the mediated electrocatalytic oxidation of reduced dihydronicotinamide adenine dinucleotide

被引：2

作者：

Antiochia, R ^{[1
]}

Lavagnini, I ^{[1
]}

Magno, F ^{[1
]}

机构：

[1] Univ Padua, Dipartimento Chim Inorgan Metallorgan & Anali, I-35171 Padua, Italy

来源：

ELECTROANALYSIS | 2003年 / 15卷 / 21期

关键词：

diaphorase; oxygen; NADH; MAP; electrocatalysis; amperometric biosensor;

D O I：

10.1002/elan.200302769

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The effect of oxygen on the mediated electrocatalytic oxidation of nicotinamide adenine dinucleotide (NADH) by diaphorase from Clostridium kluveri (DI) was studied using cyclic voltammetry, UV-vis spectrophotometry and NMR spectrometry. The enzyme was found to be able to use molecular oxygen as an electron acceptor. NADH could therefore be oxidized by two competitive electron acceptors: a redox mediator, for istance p-methylaminophenolsulfate (MAP) or ferrocene monocarboxylic acid (FMCA) properly added to the solution, and molecular oxygen. Some considerations on the consequence of the use of diaphorase from Clostridium kluveri in amperometric biosensors were also reported.

引用

页码：1713 / 1718

页数：6

共 14 条

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