Pyruvate: an in vivo marker of cestodal infestation of the human brain on proton MR spectroscopy

被引:26
作者
Jayakumar, PN [1 ]
Srikanth, SG
Chandrashekar, HS
Kovoor, JME
Shankar, SK
Anandh, B
机构
[1] Natl Inst Mental Hlth & Neurosci, Dept Neuroimaging & Intervent Radiol, Bangalore 560029, Karnataka, India
[2] Natl Inst Mental Hlth & Neurosci, Dept Neuropathol, Bangalore 560029, Karnataka, India
[3] Natl Inst Mental Hlth & Neurosci, Dept Neurosurg, Bangalore 560029, Karnataka, India
关键词
cestodes; intracranial cysts; pyruvate; MRS; in vivo spectroscopy; in vitro spectroscopy;
D O I
10.1002/jmri.10409
中图分类号
R8 [特种医学]; R445 [影像诊断学];
学科分类号
1002 ; 100207 ; 1009 ;
摘要
Purpose: To study intracranial cestodal cysts using in vivo proton magnetic resonance spectroscopy (H-1 MRS) in an effort to identify metabolite(s) that may help in recognizing the,parasitic etiology and, perhaps, viability of such tapeworm cysts, Cestodal infestations of the human central nervous system (CNS)-cysticercosis and hydatidosis - are not rare. Identification of a scolex is considered diagnostic of cysticercosis on imaging. In its absence, however, the features are non-specific. Materials and Methods: Three patients with intracranial hydatid cysts and 13, patients with intracranial cysticercal cysts, (four intraventricular, seven parenchymal, and two subarachnoid racemose cysts) were studied on a 1.5-T MR system. In Vivo H-1 MRS was performed by multivoxel two-dimensional hybrid chemical shift imaging technique (TE = 135 msec), In vitro H-1 NMR and mass spectroscopy (matrix assisted laser desorption/ionization [MALDI]) were performed on excised cysticercal and hydatid cyst fluid. MALDI spectra for pyruvate and succinate were also obtained. Results: Alanine, pyruvate, and acetate were seen in all the three hydatid cysts. Lactate was seen in racemose cysticercal cysts. A large resonance at 2.4 ppm, confirmed as pyruvate at mass spectroscopy, was seen in 13 cestodal cysts. Pyruvate was not seen in one each of racemose, intraventricular, and parenchymal cysticereal cysts. Conclusion: Pyruvate is the predominant metabolite in cestodal cysts infesting the human CNS. It may be a marker of parasitic etiology and perhaps that of viability of such intracranial cysts.
引用
收藏
页码:675 / 680
页数:6
相关论文
共 20 条
[1]  
CHANDRASHEKAR HS, 2001, P 9 AS OC C RAD SING, P414
[2]  
CHANDRASHEKAR HS, 1999, P INT C BRAIN RES NA, pC13
[3]  
Chang KH, 1998, AM J NEURORADIOL, V19, P401
[4]   Factors influencing the development and carbohydrate metabolism of Echinococcus granulosus in dogs [J].
Constantine, CC ;
Bennet-Jenkins, EM ;
Lymbery, AJ ;
Jenkins, DJ ;
Behm, CA ;
Thompson, RCA .
JOURNAL OF PARASITOLOGY, 1998, 84 (05) :873-881
[5]   Proposal of diagnostic criteria for human cysticercosis and neurocysticercosis [J].
DelBrutto, OH ;
Wadia, NH ;
Dumas, M ;
Cruz, M ;
Tsang, VCW ;
Schantz, PM .
JOURNAL OF THE NEUROLOGICAL SCIENCES, 1996, 142 (1-2) :1-6
[6]  
JAYAKUMAR PN, 2001, ADV CLIN NEUROSCI, V11, P487
[7]  
KEE HC, 1991, NEUROIMAG CLIN N AM, V1, P159
[8]   IN-VIVO PROTON MAGNETIC-RESONANCE SPECTROSCOPY IN A CASE OF INTRACRANIAL HYDATID CYST [J].
KOHLI, A ;
GUPTA, RK ;
POPTANI, H ;
ROY, R .
NEUROLOGY, 1995, 45 (03) :562-564
[9]  
MAYES PA, 1996, HARPERS BIOCH, P176
[10]   DIFFERENCES IN CHEMICAL COMPOSITION AND CARBOHYDRATE-METABOLISM OF ECHINOCOCCUS-GRANULOSUS (HORSE AND SHEEP STRAINS) AND ECHINOCOCCUS-MULTILOCULARIS [J].
MCMANUS, DP ;
SMYTH, JD .
PARASITOLOGY, 1978, 77 (AUG) :103-109