Construction of a high-biomass, iron-enriched yeast strain and study on distribution of iron in the cells of Saccharomyces cerevisiae

被引：17

作者：

Yuan, YL

Guo, XN

He, XP

Zhang, BR ^{[1
]}

Liu, SG

机构：

[1] Chinese Acad Sci, Inst Microbiol, Beijing 100080, Peoples R China

[2] Sichuan Univ, Coll Life Sci, Chengdu 610064, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2004年 / 26卷 / 04期

关键词：

biotransformation; distribution; iron content; protoplast fusion; yeast;

D O I：

10.1023/B:BILE.0000015449.30186.90

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A high-biomass, iron-enriched Saccharomyces cerevisiae ZYF-15 was constructed by interspecies protoplast fusion. Under optimal fermentation condition, the biomass and iron content of the strain reached 11 g l(-1) and 25 mg Fe g(-1) dry cells, respectively. About 96% of enriched iron is converted into organic iron, which is mainly in cell walls and vacuoles with some bound to DNA, RNA and protein.

引用

页码：311 / 315

页数：5

共 16 条

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