Overproduction, purification, crystallization and preliminary X-ray diffraction studies of the human spliceosomal protein U5-15kD

被引：7

作者：

Reuter, K ^{[1
]}

Ficner, R ^{[1
]}

机构：

[1] Univ Marburg, Inst Mol Biol & Tumorforsch, D-35037 Marburg, Germany

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 1999年 / 55卷

关键词：

D O I：

10.1107/S0907444998016151

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The gene coding for the human spliceosomal U5 snRNP-specific 15 kDa protein (U5-15kD) was overexpressed in Escherichia coli, its product purified to homogeneity and crystallized. Well diffracting single crystals were obtained by the vapour-diffusion method in hanging drops and subsequent macroseeding, The crystals belong to the orthorhombic space group P2(1)2(1)2 with a = 62.3, b = 65.7, c = 37.1 Angstrom. They diffract to at least 3.0 Angstrom and contain one molecule in the asymmetric unit. A selenomethionine derivative of the protein was prepared and crystallized for multiwavelength anomalous diffraction (MAD) data collection.

引用

页码：888 / 890

页数：3

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