Reconstitution of the Entire Hepatitis C Virus Life Cycle in Nonhepatic Cells

被引：70

作者：

Da Costa, Daniel ^{[1
,2
]}

Turek, Marine ^{[1
,2
]}

Felmlee, Daniel J. ^{[1
,2
]}

Girardi, Erika ^{[2
,3
]}

Pfeffer, Sebastien ^{[2
,3
]}

Long, Gang ^{[4
]}

Bartenschlager, Ralf ^{[4
]}

Zeisel, Mirjam B. ^{[1
,2
]}

Baumert, Thomas F. ^{[1
,2
,5
]}

机构：

[1] INSERM, U748, Strasbourg, France

[2] Univ Strasbourg, Strasbourg, France

[3] CNRS, Inst Biol Mol & Cellulaire, Architecture & Reactivite ARN, F-67084 Strasbourg, France

[4] Univ Heidelberg, Dept Mol Virol, Heidelberg, Germany

[5] Hop Univ Strasbourg, Strasbourg, France

来源：

JOURNAL OF VIROLOGY | 2012年 / 86卷 / 21期

关键词：

LIVER-SPECIFIC MICRORNA; HUMAN HEPATOCYTES; REPLICATION; INFECTION; ENTRY; ANTIBODIES; SECRETION; RNA; EXPRESSION; PARTICLES;

D O I：

10.1128/JVI.01066-12

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Hepatitis C virus (HCV) is a human hepatotropic virus, but the relevant host factors restricting HCV infection to hepatocytes are only partially understood. We demonstrate that exogenous expression of defined host factors reconstituted the entire HCV life cycle in human nonhepatic 293T cells. This study shows robust HCV entry, RNA replication, and production of infectious virus in human nonhepatic cells and highlights key host factors required for liver tropism of HCV.

引用

页码：11919 / 11925

页数：7

共 41 条

[1] Cell entry of hepatitis C virus requires a set of co-receptors that include the CD81 tetraspanin and the SR-B1 scavenger receptor [J].

Bartosch, B ;

Vitelli, A ;

Granier, C ;

Goujon, C ;

Dubuisson, J ;

Pascale, S ;

Scarselli, E ;

Cortese, R ;

Nicosia, A ;

Cosset, FL .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (43) :41624-41630

[2] Adaptation of Hepatitis C Virus to Mouse CD81 Permits Infection of Mouse Cells in the Absence of Human Entry Factors [J].

Bitzegeio, Julia ;

Bankwitz, Dorothea ;

Hueging, Kathrin ;

Haid, Sibylle ;

Brohm, Christiane ;

Zeisel, Mirjam B. ;

Herrmann, Eva ;

Iken, Marcus ;

Ott, Michael ;

Baumert, Thomas F. ;

Pietschmann, Thomas .

PLOS PATHOGENS, 2010, 6 (07) :1-15

[3] Highly permissive cell lines for subgenomic and genomic hepatitis C virus RNA replication [J].

Blight, KJ ;

McKeating, JA ;

Rice, CM .

JOURNAL OF VIROLOGY, 2002, 76 (24) :13001-13014

[4] Liver-specific MicroRNA miR-122 enhances the replication of hepatitis C virus in nonhepatic cells [J].

Chang, Jinhong ;

Cruo, Ju-Tao ;

Jiang, Dong ;

Guo, Haitao ;

Taylor, John M. ;

Block, Timothy M. .

JOURNAL OF VIROLOGY, 2008, 82 (16) :8215-8223

[5] A genetically humanized mouse model for hepatitis C virus infection [J].

Dorner, Marcus ;

Horwitz, Joshua A. ;

Robbins, Justin B. ;

Barry, Walter T. ;

Feng, Qian ;

Mu, Kathy ;

Jones, Christopher T. ;

Schoggins, John W. ;

Catanese, Maria Teresa ;

Burton, Dennis R. ;

Law, Mansun ;

Rice, Charles M. ;

Ploss, Alexander .

NATURE, 2011, 474 (7350) :208-U246

[6] Claudin-1 is a hepatitis C virus co-receptor required for a late step in entry [J].

Evans, Matthew J. ;

von Hahn, Thomas ;

Tscherne, Donna M. ;

Syder, Andrew J. ;

Panis, Maryline ;

Woelk, Benno ;

Hatziioannou, Theodora ;

McKeating, Jane A. ;

Bieniasz, Paul D. ;

Rice, Charles M. .

NATURE, 2007, 446 (7137) :801-805

[7] Viral entry and escape from antibody-mediated neutralization influence hepatitis C virus reinfection in liver transplantation [J].

Fafi-Kremer, Samira ;

Fofana, Isabel ;

Soulier, Eric ;

Carolla, Patric ;

Meuleman, Philip ;

Leroux-Roels, Geert ;

Patel, Arvind H. ;

Cosset, Francois-Loic ;

Pessaux, Patrick ;

Doffoel, Michel ;

Wolf, Philippe ;

Stoll-Keller, Francoise ;

Baumert, Thomas F. .

JOURNAL OF EXPERIMENTAL MEDICINE, 2010, 207 (09) :2019-2031

[8] Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier [J].

Fletcher, Nicola F. ;

Wilson, Garrick K. ;

Murray, Jacinta ;

Hu, Ke ;

Lewis, Andrew ;

Reynolds, Gary M. ;

Stamataki, Zania ;

Meredith, Luke W. ;

Rowe, Ian A. ;

Luo, Guangxiang ;

Lopez-Ramirez, Miguel A. ;

Baumert, Thomas F. ;

Weksler, Babette ;

Couraud, Pierre-Olivier ;

Kim, Kwang Sik ;

Romero, Ignacio A. ;

Jopling, Catherine ;

Morgello, Susan ;

Balfe, Peter ;

McKeating, Jane A. .

GASTROENTEROLOGY, 2012, 142 (03) :634-U326

[9] Hepatitis C Virus Infection of Neuroepithelioma Cell Lines [J].

Fletcher, Nicola F. ;

Yang, Jian Ping ;

Farquhar, Michelle J. ;

Hu, Ke ;

Davis, Christopher ;

He, Qiuchen ;

Dowd, Kimberly ;

Ray, Stuart C. ;

Krieger, Sophie E. ;

Neyts, Johan ;

Baumert, Thomas F. ;

Balfe, Peter ;

McKeating, Jane A. ;

Wong-Staal, Flossie .

GASTROENTEROLOGY, 2010, 139 (04) :1365-+

[10] Monoclonal Anti-Claudin 1 Antibodies Prevent Hepatitis C Virus Infection of Primary Human Hepatocytes [J].

Fofana, Isabel ;

Krieger, Sophie E. ;

Grunert, Fritz ;

Glauben, Sandra ;

Xiao, Fei ;

Fafi-Kremer, Samira ;

Soulier, Eric ;

Royer, Cathy ;

Thumann, Christine ;

Mee, Christopher J. ;

McKeating, Jane A. ;

Dragic, Tatjana ;

Pessaux, Patrick ;

Stoll-Keller, Francoise ;

Schuster, Catherine ;

Thompson, John ;

Baumert, Thomas F. .

GASTROENTEROLOGY, 2010, 139 (03) :953-U339

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