Development of a triplex PCR assay for the specific detection of Campylobacter jejuni, Salmonella spp., and Escherichia coli O157:H7

被引：34

作者：

Gilbert, C ^{[1
]}

Winters, D ^{[1
]}

O'Leary, A ^{[1
]}

Slavik, M ^{[1
]}

机构：

[1] Univ Arkansas, Ctr Excellence Poultry Sci, Dept Poultry Sci, Fayetteville, AR 72701 USA

来源：

MOLECULAR AND CELLULAR PROBES | 2003年 / 17卷 / 04期

关键词：

Campylobacter jejuni; Escherichia coli O157 : H7; Salmonella; multiplex PCR; detection;

D O I：

10.1016/S0890-8508(03)00043-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A triplex PCR assay was developed and evaluated for efficacy in detecting Campylobacter jejuni, Salmonella spp., and Escherichia coli O157:H7 in a variety of raw and ready-to-eat food products. Following a short enrichment period, artificially contaminated food samples were subjected to a triplex PCR assay, which incorporated published primers for each food pathogen, a protocol for sample collection, and a PCR procedure designed specifically for the assay. The selected primers amplified fragment sizes of 159 bp, 252 bp, and 360 by for C. jejuni, E. coli O157:H7, and Salmonella spp., respectively. This assay provides specific and reliable results and allows for the cost-effective detection of all three bacterial pathogens in one reaction tube. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：135 / 138

页数：4

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