Formation of a DNA/polygalactosamine complex and its interaction with cells

被引：19

作者：

Sato, T ^{[1
]}

Shirakawa, N ^{[1
]}

Nishi, H ^{[1
]}

Okahata, Y ^{[1
]}

机构：

[1] TOKYO INST TECHNOL, DEPT BIOMOL ENGN, MIDORI KU, YOKOHAMA, KANAGAWA 226, JAPAN

来源：

CHEMISTRY LETTERS | 1996年 / 09期

关键词：

DNA; EFFICIENT; GENE;

D O I：

10.1246/cl.1996.725

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

DNA complexes with naturally occurring polysaccharides, polygalactosamine or chitosan, were formed in water. Thermal profiles, CD spectrum, zeta-potentials, and cell uptake were investigated. The DNA/polygalactosamine complex showed higher cell uptake than DNA/chitosan complex did.

引用

收藏

页码：725 / 726

页数：2

相关论文

共 12 条

[1]

Akashi M., 1989, J BIOACT COMPAT POLY, V4, P124

[2] EFFICIENT GENE-TRANSFER INTO MAMMALIAN PRIMARY ENDOCRINE-CELLS WITH LIPOPOLYAMINE-COATED DNA [J].

BEHR, JP ;

DEMENEIX, B ;

LOEFFLER, JP ;

MUTUL, JP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (18) :6982-6986

[3] LIPOFECTION - A HIGHLY EFFICIENT, LIPID-MEDIATED DNA-TRANSFECTION PROCEDURE [J].

FELGNER, PL ;

GADEK, TR ;

HOLM, M ;

ROMAN, R ;

CHAN, HW ;

WENZ, M ;

NORTHROP, JP ;

RINGOLD, GM ;

DANIELSEN, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (21) :7413-7417

[4] ANTITUMOR-ACTIVITY OF POLYGALACTOSAMINE ISOLATED FROM PAECILOMYCES SP I-1 STRAIN [J].

ISHITANI, K ;

SUZUKI, S ;

SUZUKI, M .

JOURNAL OF PHARMACOBIO-DYNAMICS, 1988, 11 (01) :58-65

[5]

LEMAITRE JP, 1990, P NATL ACAD SCI USA, V84, P648

[6]

MONSIGNY M, 1983, BIOL CELL, V47, P95

[7] ELECTROPORATION - APPLICATION TO HUMAN LYMPHOID-CELL LINES FOR STABLE INTRODUCTION OF A TRANSACTIVATOR GENE OF HUMAN T-CELL LEUKEMIA-VIRUS TYPE-I [J].

OHTANI, K ;

NAKAMURA, M ;

SAITO, S ;

NAGATA, K ;

SUGAMURA, K ;

HINUMA, Y .

NUCLEIC ACIDS RESEARCH, 1989, 17 (04) :1589-1604

[8] FORMATION OF A DNA COMPLEX WITH LIPOGLUTAMIDE HAVING TETRAETHYLENEGLYCOL TAILS AND ITS INTERACTION WITH A TUMOR-CELL [J].

SATO, T ;

AKINO, H ;

OKAHATA, Y .

CHEMISTRY LETTERS, 1995, (09) :755-756

[9] PREPARATION AND CHARACTERIZATION OF DNA-LIPOGLUTAMATE COMPLEXES [J].

SATO, T ;

KAWAKAMI, T ;

SHIRAKAWA, N ;

OKAHATA, Y .

BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 1995, 68 (09) :2709-2715

[10] RECENT ASPECTS IN THE USE OF LIPOSOMES IN BIOTECHNOLOGY AND MEDICINE [J].

SATO, T ;

SUNAMOTO, J .

PROGRESS IN LIPID RESEARCH, 1992, 31 (04) :345-372