Elevated estrogen receptor expression in hypothalamic preoptic area decreased by electroacupuncture in ovariectomized rats

被引：31

作者：

Ma, Shulan ^{[1
,2
]}

Wu, Jihong ^{[3
]}

Feng, Yi ^{[1
]}

Chen, Boying ^{[1
]}

机构：

[1] Fudan Univ, Shanghai Med Coll, State Key Lab Med Neurobiol,Inst Brain Sci, Dept Integrat Med & Neurobiol,WHO Collaborating C, Shanghai 200433, Peoples R China

[2] Fudan Univ, Shanghai Med Coll, Teaching Ctr Expt Med, Shanghai 200433, Peoples R China

[3] Fudan Univ, EYE & ENT Hosp, Expt Res Ctr, Shanghai 200433, Peoples R China

来源：

NEUROSCIENCE LETTERS | 2011年 / 494卷 / 02期

关键词：

Electroacupuncture; Ovariectomized rats; Hypothalamic preoptic area; Estrogen receptors; HORMONE NEURONS; MEDIAN-EMINENCE; FEMALE RAT; ALPHA; ACUPUNCTURE; BRAIN; IMMUNOREACTIVITY; CELLS; AXIS;

D O I：

10.1016/j.neulet.2011.02.070

中图分类号：

Q189 [神经科学];

学科分类号：

071006 [神经生物学];

摘要：

In the present study, effects of electroacupuncture (EA) on estrogen receptor alpha (ER alpha) and beta (ER beta) mRNA and protein expression in the hypothalamus of ovariectomized (OVX) rats were detected by quantitative real-time reverse transcription PCR (qRT-PCR) and western blot analysis. Gonadotropin-releasing hormone (GnRH) release and GnRH mRNA level in hypothalamic preoptic area (POA) were evaluated by push-pull perfusion and qRT-PCR. Our results showed that elevated mRNA and protein expression of ER alpha and ER beta in hypothalamus were restrained following EA treatment in OVX rats. EA treatment also inhibited GnRH release and GnRH mRNA levels in OVX rats. These results provide novel evidence that EA treatment down regulates the expression of hypothalamic estrogen receptors (ERs), thus restores the response of GnRH neurons to estrogen depression, and partially resets the negative feedback of estrogen to hypothalamus-pituitary-ovary axis (HPOA) in OVX rats, which may be a critical mechanism for EA on female reproductive disorders. (C) 2011 Elsevier Ireland Ltd. All rights reserved.

引用

页码：109 / 113

页数：5

共 26 条

[1]

Butler JA, 1999, J NEUROENDOCRINOL, V11, P331

[2]

Stereologic analysis of estrogen receptor alpha (ERα) expression in rat hypothalamus and its regulation by aging and estrogen [J].