Domain structure of separase and its binding to securin as determined by EM

被引：39

作者：

Viadiu, H

Stemmann, O

Kirschner, MW

Walz, T

机构：

[1] Harvard Univ, Sch Med, Dept Cell Biol, Boston, MA 02115 USA

[2] Harvard Univ, Sch Med, Dept Syst Biol, Boston, MA 02115 USA

[3] Max Planck Inst Biochem, Dept Mol Cell Biol, D-82152 Martinsried, Germany

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2005年 / 12卷 / 06期

关键词：

D O I：

10.1038/nsmb935

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

After the degradation of its inhibitor securin, separase initiates chromosome segregation during the metaphase-to-anaphase transition by cleaving cohesin. Here we present a density map at a resolution of 25 angstrom of negatively stained separase-securin complex. Based on labeling data and sequence analysis, we propose a model for the structure of separase, consisting of 26 ARM repeats, an unstructured region of 280 residues and two caspase-like domains, with securin binding to the ARM repeats.

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页码：552 / 553

页数：2

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