Formalization of the MESF unit of fluorescence intensity

被引:97
作者
Schwartz, A
Gaigalas, AK
Wang, LL
Marti, GE
Vogt, RF
Fernandez-Repollet, E
机构
[1] Univ Puerto Rico, Sch Med, Dept Pharmacol, San Juan, PR 00936 USA
[2] Ctr Dis Control, Div Sci Lab, Atlanta, GA 30333 USA
[3] US FDA, Ctr Biol Evaluat & Res, Bethesda, MD USA
[4] NIST, Div Biotechnol, Gaithersburg, MD 20899 USA
[5] Ctr Quantitat Cytometry, San Juan, PR USA
关键词
MESF; Molecules of Equivalent Soluble Fluorochrome; quantitation; fluorescence intensity;
D O I
10.1002/cyto.b.10066
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
This report summarizes the work performed during the past two years at the National Institute of Standards and Technology (NIST) in the refinement and formal definition of the MESF unit of fluorescence intensity. In addition to the theory underlying the MESF unit, considerations of error analysis are also presented. The details of this work may be found in the three publications of the NIST Journal of Research (www.nist.gov) listed as the references 2-4. The use of the fluorescence intensity unit provides a tool to compare quantitative fluorescence intensity measurements over time and across platforms. (C) 2003 Wiley-Liss, Inc.
引用
收藏
页码:1 / 6
页数:6
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