Analysis of omeprazole, midazolam and hydroxy-metabolites in plasma using liquid chromatography coupled to tandem mass spectrometry

被引：28

作者：

Frerichs, VA ^{[1
]}

Zaranek, C ^{[1
]}

Haas, CE ^{[1
]}

机构：

[1] SUNY Buffalo, Dept Pharm Practice, Pharmacotherapy Res Ctr, Core Analyt Lab, Buffalo, NY 14260 USA

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2005年 / 824卷 / 1-2期

关键词：

omeprazole; midazolam; analysis; LCMS; CYP2C19;

D O I：

10.1016/j.jchromb.2005.07.001

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method has been developed and validated for the quantitation of midazolam, alphahydroxy-midazolam, omeprazole, and hydroxyomeprazole from one 250 mu L sample of human plasma using high performance liquid chromatography coupled to tandem mass spectrometry. The method was validated for a daily working range of 0.400-100 ng/mL, with limits of detection between 2 and 15 pg/mL. The inter-assay variation was less than 15% for all analytes at four control concentrations and the samples were stable for three freeze-thaw cycles under the analysis conditions and 24 h in the post-preparative analysis matrix. This method was used to analyze samples in support of clinical studies probing the activity of the cytochrome P-450 enzyme system. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：71 / 80

页数：10

共 26 条

[1]

*AM CHEM SOC, 2004, SCIF SCHOL ONL 2004

[2] PHOTOCHEMICAL DECOMPOSITION OF MIDAZOLAM .4. STUDY OF PH-DEPENDENT STABILITY BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].