Selection of B lymphocytes in the periphery is determined by the functional capacity of the B cell antigen receptor

被引:12
作者
Wang, LD
Lopes, J
Cooper, AB
Dang-Lawson, M
Matsuuchi, L
Clark, MR
机构
[1] Rheumatol Sect, Chicago, IL 60637 USA
[2] Univ Chicago, Comm Immunol, Div Biol Sci, Chicago, IL 60637 USA
[3] Univ Chicago, Pritzker Sch Med, Chicago, IL 60637 USA
[4] Univ British Columbia, Dept Zool, Cell Biol Grp, Vancouver, BC V6T 1Z4, Canada
关键词
signal transduction; B cell differentiation; CD antigen; tyrosine kinase;
D O I
10.1073/pnas.0307040101
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Within the B cell antigen receptor (BCR), the cytoplasmic tails of both Igalpha and Igbeta are required for normal B cell development and maturation. To dissect the mechanisms by which each tail contributes to development in vivo, Igbeta(-1-) mice were reconstituted with retroviruses encoding either wild-type Igbeta, an Igbeta molecule lacking a cytoplasmic tail (Igbeta(Deltac)) or one in which the cytoplasmic tail was derived from lgalpha (Igbeta(calpha)). All constructs rescued B cell development and generated immature B cell populations in the bone marrow with similar expression levels of both Igbeta and membrane-bound IgM. In the periphery, receptor-surface density was inversely proportional to the number of Igalpha tails in the BCR. Although peripheral-surface-receptor levels differed, splenic B cells expressing either Igbeta or Igbeta(calpha) responded similarly to stimulation through the BCR. Analysis of membrane-bound IgM and Igbeta expression revealed that peripheral-receptor expression was primarily determined by positive selection between the bone marrow and peripheral immature B cell populations. These data indicate that B cells are selected into the periphery on the basis of a common level of antigen responsiveness.
引用
收藏
页码:1027 / 1032
页数:6
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