The long isoform of the cell adhesion molecule C-CAM binds to actin

C-CAM is a member of the carcinoembryonic antigen family (CEA) of the rat, which mediates cell adhesion in vitro and binds to signal transduction molecules. In many tissues C-CAM is expressed in the apical domain of the plasma membrane in close contact with intracellular cortical microfilaments, e.g., in the microvilli of the brush borders of enterocytes, Regarding this subcellular localisation, we have investigated the C-CAM interaction with the cytoskeleton. The association of C-CAM with detergent-insoluble structures increased when the small intestinal mucosa was extracted under conditions known to preserve the cytoskeleton of the brush borders. We found a coimmunoprecipitation of actin with C-CAM of the small intestine mucosa which increased in the presence of the chemical cross-linker DSP, allowing the demonstration of complexes between C-CAM and actin of different molecular masses, A recombinant fusion protein of the cytoplasmic domain of the long isoform of C-CAM bound specifically to purified actin in a cosedimentation assay. These results suggest an intrinsic actin-binding activity of C-CAM. (C) 1999 Academic Press.