A simple and efficient method to transcribe RNAs with reduced 3′ heterogeneity

被引：58

作者：

Kao, C ^{[1
]}

Rüdisser, S

Zheng, MX

机构：

[1] Indiana Univ, Dept Biol, Bloomington, IN 47405 USA

[2] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA

[3] Lawrence Berkeley Lab, Struct Biol Div, Berkeley, CA 94720 USA

来源：

METHODS | 2001年 / 23卷 / 03期

关键词：

D O I：

10.1006/meth.2000.1131

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Ribose C2 ' methoxy groups (-OCH3) on the penultimate nucleotide or the last two nucleotides of DNA templates can dramatically improve the quality of transcripts produced by T7 RNA polymerase. This strategy can be adapted to generate transcripts of varying lengths and will allow greater ease in purification of the products. (C) 2001 Academic Press.

引用

页码：201 / 205

页数：5

共 19 条

[1]

AUSUBEL FM, 1994, CURRENT PROTOCLES MO

[2]

DIANZOTT AM, 1988, NUCLEIC ACIDS RES, V16, P10940

[3] OMPT ENCODES THE ESCHERICHIA-COLI OUTER-MEMBRANE PROTEASE THAT CLEAVES T7-RNA POLYMERASE DURING PURIFICATION [J].