Expression and functional analysis of a novel Fusion Competent Myoblast specific GAL4 driver

被引：7

作者：

Beckett, Karen ^{[1
]}

Rochlin, Kate M. ^{[2
]}

Duan, Hong ^{[3
,4
]}

Nguyen, Hanh T. ^{[3
,4
]}

Baylies, Mary K. ^{[1
,2
]}

机构：

[1] Mem Sloan Kettering Canc Ctr, Program Dev Biol, New York, NY 10021 USA

[2] Cornell Univ, Weill Grad Sch Med Sci, BCMB Program, New York, NY 10021 USA

[3] Albert Einstein Coll Med, Dept Med, Bronx, NY 10461 USA

[4] Albert Einstein Coll Med, Dept Dev & Mol Biol, Bronx, NY 10461 USA

来源：

GENE EXPRESSION PATTERNS | 2008年 / 8卷 / 02期

关键词：

muscle; fusion competent myoblast; myotube; drosophila; dmef2; lmd; rac; myoblast; fusion; GAL4;

D O I：

10.1016/j.modgep.2007.10.002

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In the Drosophila embryo, body wall muscles are formed by the fusion of two cell types, Founder Cells (FCs) and Fusion Competent Myoblasts (FCMs). Using an enhancer derived from the Dmef2 gene ([C/D]*), we report the first GAL4 driver specifically expressed in FCMs. We have determined that this GAL4 driver causes expression in a subset of FCMs and, upon fusion, in developing myotubes from stage 14 onwards. In addition, we have shown that using this Dmef2-5x[C/D]*-GAL4 driver to express dominant negative Rac in only FCMs causes a partial fusion block. This novel GAL4 driver will provide a useful reagent to study Drosophila myoblast fusion and muscle differentiation. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：87 / 91

页数：5

共 22 条

[1]

BATE M, 1990, DEVELOPMENT, V110, P791

[2] Myogenesis:: A view from Drosophila [J].

Baylies, MK ;

Bate, M ;

Gomez, MR .

CELL, 1998, 93 (06) :921-927

[3]

BECKETT K, UNPUB EGRF INTEGRINS

[4] 3D analysis of founder cell and fusion competent myoblast arrangements outlines a new model of myoblast fusion [J].

Beckett, Karen ;

Baylies, Mary K. .

DEVELOPMENTAL BIOLOGY, 2007, 309 (01) :113-125

[5] Parcas, a regulator of non-receptor tyrosine kinase signaling, acts during anterior-posterior patterning and somatic muscle development in Drosophila melanogaster [J].

Beckett, Karen ;

Baylies, Mary K. .

DEVELOPMENTAL BIOLOGY, 2006, 299 (01) :176-192

[6] The development of the Drosophila larval body wall muscles [J].

Beckett, Karen ;

Baylies, Mary K. .

FLY NEUROMUSCULAR JUNCTION: STRUCTURE AND FUNCTION, SECOND EDITION, 2006, 75 :55-70

[7] DROSOPHILA MEF2, A TRANSCRIPTION FACTOR THAT IS ESSENTIAL FOR MYOGENESIS [J].

BOUR, BA ;

OBRIEN, MA ;

LOCKWOOD, WL ;

GOLDSTEIN, ES ;

BODMER, R ;

TAGHERT, PH ;

ABMAYR, SM ;

NGUYEN, HT .

GENES & DEVELOPMENT, 1995, 9 (06) :730-741

[8] The myogenic regulatory gene Mef2 is a direct target for transcriptional activation by Twist during Drosophila myogenesis [J].

Cripps, RM ;

Black, BL ;

Zhao, B ;

Lien, CL ;

Schulz, RA ;

Olson, EN .

GENES & DEVELOPMENT, 1998, 12 (03) :422-434

[9]

Duan H, 2001, DEVELOPMENT, V128, P4489

[10] Patterns of gene expression during Drosophila mesoderm Development [J].

Furlong, EEM ;

Andersen, EC ;

Null, B ;

White, KP ;

Scott, MP .

SCIENCE, 2001, 293 (5535) :1629-1633

← 1 2 3 →