Cloning, characterization and functional analysis of groEL-like gene from thermophilic cyanobacterium Synechococcus vulcanus, which does not form an operon with groES

被引:27
作者
Furuki, M [1 ]
Tanaka, N [1 ]
Hiyama, T [1 ]
Nakamoto, H [1 ]
机构
[1] SAITAMA UNIV, DEPT CHEM & MOL BIOL, URAWA, SAITAMA 338, JAPAN
来源
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY | 1996年 / 1294卷 / 02期
关键词
heat shock protein; groEL gene; thermophile; cyanobacterium; GroEL protein; chaperonin;
D O I
10.1016/0167-4838(96)00037-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A gene encoding 57 102 Da polypeptide homologous to groEL of Escherichia coli but accompanying no groES, has been cloned and sequenced from a thermophilic cyanobacterium, Synechococcus vulcanus. The amount of the gene transcript increased several folds by heat shock. The gene was expressed as a minor component of two types of HSP60, and designated as groEL2. Although expressed and induced well upon heat shock treatment in the E. coli, introduction of the cloned groEL2 gene of S. vulcanus into an E. coli groEL-less mutant did not result in the complementation of heat sensitivity.
引用
收藏
页码:106 / 110
页数:5
相关论文
共 20 条
[1]  
AIBA H, 1981, J BIOL CHEM, V256, P1905
[2]  
CHITNIS PR, 1991, J BIOL CHEM, V266, P58
[3]  
DEWIT TFR, 1992, MOL MICROBIOL, V6, P1995
[4]  
GATENBY AA, 1994, ANNU REV PLANT PHYS, V45, P469
[5]   HOMOLOGOUS PLANT AND BACTERIAL PROTEINS CHAPERONE OLIGOMERIC PROTEIN ASSEMBLY [J].
HEMMINGSEN, SM ;
WOOLFORD, C ;
VANDERVIES, SM ;
TILLY, K ;
DENNIS, DT ;
GEORGOPOULOS, CP ;
HENDRIX, RW ;
ELLIS, RJ .
NATURE, 1988, 333 (6171) :330-334
[6]   UNIDIRECTIONAL DIGESTION WITH EXONUCLEASE-III CREATES TARGETED BREAKPOINTS FOR DNA SEQUENCING [J].
HENIKOFF, S .
GENE, 1984, 28 (03) :351-359
[7]  
KATOH S, 1993, METHOD ENZYMOL, V167, P263
[8]   EFFECTS OF MUTATIONS IN HEAT-SHOCK GENES GROES AND GROEL ON PROTEIN EXPORT IN ESCHERICHIA-COLI [J].
KUSUKAWA, N ;
YURA, T ;
UEGUCHI, C ;
AKIYAMA, Y ;
ITO, K .
EMBO JOURNAL, 1989, 8 (11) :3517-3521
[9]  
LEHEL C, 1993, J BIOL CHEM, V268, P1799
[10]  
LEVIN B, 1994, GENE, V5