A role for the COUP-TF-related gene seven-up in the diversification of cardioblast identities in the dorsal vessel of Drosophila

被引:144
作者
Lo, PCH [1 ]
Frasch, M [1 ]
机构
[1] Mt Sinai Sch Med, Dept Biochem & Mol Biol, New York, NY 10029 USA
关键词
Drosophila heart; tinman; seven-up; Cardioblasts;
D O I
10.1016/S0925-4773(01)00361-6
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The Drosophila gene tinman is essential for dorsal vessel (heart) formation and is structurally and functionally conserved in vertebrates. In the mature embryonic dorsal vessel, tinman is expressed in four of the six pairs of cardioblasts in each segment. We provide evidence that seven-lip, which is homologous to the vertebrate COUP-TF transcription factor and is expressed in the non-Tinman-expressing cardioblasts, represses tinman in these cells. Loss of function seven-lip mutations derepress tinman expression in these cardioblasts while ectopic expression of seven-np represses tinman in the cardioblasts that normally express it. These changes are correlated with alterations in the expression of additional molecular markers for each of these two types of cardioblasts, such as the novel T-box-containing gene Tb66F2 and the potassium channel-encoding gene sur. These observations suggest that seven-up has a role in diversifying cardioblast identities within each segment. We also describe the tin,nan cis sequences that mediate tinman repression by seven-hip and examine whether Seven-up can bind these sequences to directly inhibit tinman. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:49 / 60
页数:12
相关论文
共 47 条
[1]   Toward understanding the assembly and structure of KATP channels [J].
Aguilar-Bryan, L ;
Clement, JP ;
Gonzalez, G ;
Kunjilwar, K ;
Babenko, A ;
Bryan, J .
PHYSIOLOGICAL REVIEWS, 1998, 78 (01) :227-245
[2]   TINMAN AND BAGPIPE - 2 HOMEO BOX GENES THAT DETERMINE CELL FATES IN THE DORSAL MESODERM OF DROSOPHILA [J].
AZPIAZU, N ;
FRASCH, M .
GENES & DEVELOPMENT, 1993, 7 (7B) :1325-1340
[3]   Mutations in human cause limb and cardiac malformation in Holt-Oram syndrome [J].
Basson, CT ;
Bachinsky, DR ;
Lin, RC ;
Levi, T ;
Elkins, JA ;
Soults, J ;
Grayzel, D ;
Kroumpouzou, E ;
Traill, TA ;
LeblancStraceski, J ;
Renault, B ;
Kucherlapati, R ;
Seidman, JG ;
Seidman, CE .
NATURE GENETICS, 1997, 15 (01) :30-35
[4]  
BEGEMANN G, 1995, DEVELOPMENT, V121, P225
[5]  
BODMER R, 1993, DEVELOPMENT, V118, P719
[6]  
Bodmer R., 1999, HEART DEV
[7]   DROSOPHILA MEF2, A TRANSCRIPTION FACTOR THAT IS ESSENTIAL FOR MYOGENESIS [J].
BOUR, BA ;
OBRIEN, MA ;
LOCKWOOD, WL ;
GOLDSTEIN, ES ;
BODMER, R ;
TAGHERT, PH ;
ABMAYR, SM ;
NGUYEN, HT .
GENES & DEVELOPMENT, 1995, 9 (06) :730-741
[8]  
BRAND AH, 1993, DEVELOPMENT, V118, P401
[9]   Genetics of heart development [J].
Chen, JN ;
Fishman, MC .
TRENDS IN GENETICS, 2000, 16 (09) :383-388
[10]   Vertebrate tinman homologues and cardiac differentiation [J].
Evans, SM .
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, 1999, 10 (01) :73-83