Who is who in litter decomposition? Metaproteomics reveals major microbial players and their biogeochemical functions

被引:527
作者
Schneider, Thomas [1 ]
Keiblinger, Katharina M. [2 ]
Schmid, Emanuel [1 ]
Sterflinger-Gleixner, Katja [3 ]
Ellersdorfer, Guenther [3 ]
Roschitzki, Bernd [4 ,5 ]
Richter, Andreas [6 ,7 ]
Eberl, Leo [1 ]
Zechmeister-Boltenstern, Sophie [2 ]
Riedel, Kathrin [1 ,8 ]
机构
[1] Univ Zurich, Inst Plant Biol, Dept Microbiol, CH-8008 Zurich, Switzerland
[2] Univ Nat Resources & Life Sci BOKU, Dept Forest & Soil Sci, Inst Soil Res, Vienna, Austria
[3] Univ Nat Resources & Life Sci BOKU, Dept Biotechnol, Inst Appl Microbiol, Vienna, Austria
[4] Univ Zurich, Funct Genom Ctr, CH-8008 Zurich, Switzerland
[5] Swiss Fed Inst Technol, Zurich, Switzerland
[6] Univ Vienna, Vienna, Austria
[7] Univ Western Australia, Sch Earth & Environm, Perth, WA 6009, Australia
[8] Inst Microbiol, Greifswald, Germany
基金
奥地利科学基金会;
关键词
extracellular enzymes; leaf-litter decomposition; litter nutrient content; metaproteomics; microbial ecology; microbial succession; SOIL BACTERIAL COMMUNITIES; LEAF-LITTER; TERRESTRIAL ECOSYSTEMS; MASS-SPECTROMETRY; ENZYME-ACTIVITY; ORGANIC-MATTER; FUNGAL BIOMASS; FOREST SOILS; STOICHIOMETRY; NITROGEN;
D O I
10.1038/ismej.2012.11
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
Leaf-litter decomposition is a central process in carbon cycling; however, our knowledge about the microbial regulation of this process is still scarce. Metaproteomics allows us to link the abundance and activity of enzymes during nutrient cycling to their phylogenetic origin based on proteins, the 'active building blocks' in the system. Moreover, we employed metaproteomics to investigate the influence of environmental factors and nutrients on the decomposer structure and function during beech litter decomposition. Litter was collected at forest sites in Austria with different litter nutrient content. Proteins were analyzed by 1-D-SDS-PAGE followed by liquid-chromatography and tandem mass-spectrometry. Mass spectra were assigned to phylogenetic and functional groups by a newly developed bioinformatics workflow, assignments being validated by complementary approaches. We provide evidence that the litter nutrient content and the stoichiometry of C:N:P affect the decomposer community structure and activity. Fungi were found to be the main producers of extracellular hydrolytic enzymes, with no bacterial hydrolases being detected by our metaproteomics approach. Detailed investigation of microbial succession suggests that it is influenced by litter nutrient content. Microbial activity was stimulated at higher litter nutrient contents via a higher abundance and activity of extracellular enzymes. The ISME Journal (2012) 6, 1749-1762; doi:10.1038/ismej.2012.11; published online 8 March 2012 Subject Category: microbial ecology and functional diversity of natural habitats
引用
收藏
页码:1749 / 1762
页数:14
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