Construction of a cDNA microarray derived from the ascidian Ciona intestinalis

被引：28

作者：

Azumi, K ^{[1
]}

Takahashi, H

Miki, Y

Fujie, M

Usami, T

Ishikawa, H

Kitayama, A

Satou, Y

Ueno, N

Satoh, N

机构：

[1] Hokkaido Univ, Grad Sch Pharmaceut Sci, Dept Biochem, Sapporo, Hokkaido 0600812, Japan

[2] Natl Inst Basic Biol, Dev Biol Lab, Okazaki, Aichi 4448585, Japan

[3] Kyoto Univ, Grad Sch Sci, Dept Zool, Kyoto 6068502, Japan

[4] Japan Sci & Technol Corp, CREST, Tokyo 1030027, Japan

来源：

ZOOLOGICAL SCIENCE | 2003年 / 20卷 / 10期

关键词：

ascidian; Ciona intestinalis; cDNA microarray; gene expression; development;

D O I：

10.2108/zsj.20.1223

中图分类号：

Q95 [动物学];

学科分类号：

071002 [动物学];

摘要：

A cDNA microarray was constructed from a basal chordate, the ascidian Ciona intestinalis. The draft genome of Ciona has been read and inferred to contain similar to16,000 protein-coding genes, and cDNAs for transcripts of 13,464 genes have been characterized and compiled as the "Ciona intestinalis Gene Collection Release I". In the present study, we constructed a cDNA microarray of these 13,464 Ciona genes. A preliminary experiment with Cy3- and Cy5-labeled probes showed extensive differential gene expression between fertilized eggs and larvae. In addition, there was a good correlation between results obtained by the present microarray analysis and those from previous EST analyses. This first microarray of a large collection of Ciona intestinalis cDNA clones should facilitate the analysis of global gene expression and gene networks during the embryogenesis of basal chordates.

引用

页码：1223 / 1229

页数：7

共 30 条

[1]

Gene expression during the life cycle of Drosophila melanogaster [J].

Arbeitman, MN ;

Furlong, EEM ;

Imam, F ;

Johnson, E ;

Null, BH ;

Baker, BS ;

Krasnow, MA ;

Scott, MP ;

Davis, RW ;

White, KP .

SCIENCE, 2002, 297 (5590) :2270-2275

[2]

LOCALLY WEIGHTED REGRESSION - AN APPROACH TO REGRESSION-ANALYSIS BY LOCAL FITTING [J].

CLEVELAND, WS ;

DEVLIN, SJ .

JOURNAL OF THE AMERICAN STATISTICAL ASSOCIATION, 1988, 83 (403) :596-610

[3]

Conklin E.G., 1905, J ACAD NATL SCI PHIL, V13, P1, DOI DOI 10.5962/BHL.TITLE.4801

[4]

Corbo JC, 1997, DEVELOPMENT, V124, P589

[5]

The ascidian as a model organism in developmental and evolutionary biology [J].

Corbo, JC ;

Di Gregorio, A ;

Levine, M .

CELL, 2001, 106 (05) :535-538

[6]

The draft genome of Ciona intestinalis:: Insights into chordate and vertebrate origins [J].

Dehal, P ;

Satou, Y ;

Campbell, RK ;

Chapman, J ;

Degnan, B ;

De Tomaso, A ;

Davidson, B ;

Di Gregorio, A ;

Gelpke, M ;

Goodstein, DM ;

Harafuji, N ;

Hastings, KEM ;

Ho, I ;

Hotta, K ;

Huang, W ;

Kawashima, T ;

Lemaire, P ;

Martinez, D ;

Meinertzhagen, IA ;

Necula, S ;

Nonaka, M ;

Putnam, N ;

Rash, S ;

Saiga, H ;

Satake, M ;

Terry, A ;

Yamada, L ;

Wang, HG ;

Awazu, S ;

Azumi, K ;

Boore, J ;

Branno, M ;

Chin-bow, S ;

DeSantis, R ;

Doyle, S ;

Francino, P ;

Keys, DN ;

Haga, S ;

Hayashi, H ;

Hino, K ;

Imai, KS ;

Inaba, K ;

Kano, S ;

Kobayashi, K ;

Kobayashi, M ;

Lee, BI ;

Makabe, KW ;

Manohar, C ;

Matassi, G ;

Medina, M .

SCIENCE, 2002, 298 (5601) :2157-2167

[7]

Gene expression profiles in Ciona intestinalis cleavage-stage embryos [J].

Fujiwara, S ;

Maeda, Y ;

Shin-I, T ;

Kohara, Y ;

Takatori, N ;

Satou, Y ;

Satoh, N .

MECHANISMS OF DEVELOPMENT, 2002, 112 (1-2) :115-127

[8]

Patterns of gene expression during Drosophila mesoderm Development [J].

Furlong, EEM ;

Andersen, EC ;

Null, B ;

White, KP ;

Scott, MP .

SCIENCE, 2001, 293 (5535) :1629-1633

[9]

Genomic analysis of gene expression in C-elegans [J].

Hill, AA ;

Hunter, CP ;

Tsung, BT ;

Tucker-Kellogg, G ;

Brown, EL .

SCIENCE, 2000, 290 (5492) :809-812

[10]

HOLLAND PWH, 1994, DEVELOPMENT, P125

← 1 2 3 →