Effects of initial length on intrinsic tone in guinea pig tracheal smooth muscle

In the guinea pig, tracheal smooth muscle (TSM) exhibits intrinsic tone (IT). The active nature of IT suggests that it could be influenced by muscle length and load. In the guinea pig, IT is entirely suppressed by the cyclooxygenase inhibitor indomethacin. IT could be measured as the difference between resting tone before and after indomethacin addition. We examined, in electrically stimulated TSM strips (n = 9), the influence of initial muscle length (L-i) on IT, the relationship between IT and the maximum extent of relaxation (Delta F-1), and the influence of indomethacin on active isometric force. When L-i decreased from 100 to 75% of optimal L-i, there was a significant decrease in IT (from 12.0 +/- 0.2 to 5.3 +/- 0.1 mN; P < 0.001). Over the range of L-i studied, Delta F-1 underestimated the amount of IT, but there was a close linear relationship between Delta F-1 and IT (r = 0.9). Compared with the basal state, indomethacin increased active isometric force (from 9.5 +/- 1.0 to 19.7 +/- 2.0 mN at optimal L-i; P < 0.001) and induced its length dependency. In guinea pig TSM, L-i was an important determinant of IT.