A new plasmid vector for regulated gene expression in Bacillus subtilis

被引：16

作者：

Serrano-Heras, G ^{[1
]}

Salas, M ^{[1
]}

Bravo, A ^{[1
]}

机构：

[1] Univ Autonoma Madrid, CSIC, Ctr Biol Mol Severo Ochoa, Inst Biol Mol Eladio Vinuela, E-28049 Madrid, Spain

来源：

PLASMID | 2005年 / 54卷 / 03期

关键词：

Bacillus subtilis; plasmid vector; regulated gene expression;

D O I：

10.1016/j.plasmid.2005.04.002

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have developed a novel regulated expression vector for Bacillus subtilis based on the Staphylococcus aureus plasmid pUB110. This vector, named pPR54, carries the P-R promoter and the cI857 gene (encoding a temperature-sensitive transcriptional repressor) from the Escherichia coli phage lambda. Using the gfp gene from the jellyfish Aequorea victoria as a reporter, we show that pPR54 is a useful vector for controllable production of heterologous proteins in B. subtilis. (c) 2005 Elsevier Inc. All rights reserved.

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页码：278 / 282

页数：5

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