Reduced nonspecific adsorption on covalently immobilized protein surfaces using poly(ethylene oxide) containing blocking agents

被引:106
作者
Frederix, F
Bonroy, K
Reekmans, G
Laureyn, W
Campitelli, A
Abramov, MA
Dehaen, W
Maes, G
机构
[1] IMEC, MCP, BIO, B-3001 Heverlee, Belgium
[2] Katholieke Univ Leuven, Dept Chem, B-3001 Louvain, Belgium
来源
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 2004年 / 58卷 / 01期
关键词
nonspecific adsorption; covalent protein coupling; self-assembled monolayers; surface plasmon resonance; poly(ethylene oxide);
D O I
10.1016/S0165-022X(03)00150-7
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
In a number of applications, e.g. DNA/protein micro-array technology, enzyme-linked immunosorbent assay (ELISA) technology or surface plasmon resonance (SPR) technology, the covalent coupling of proteins to surfaces is required. Following the covalent coupling of proteins, the remaining reactive groups should be blocked in order to avoid covalent binding of the analyte to the reactive surface. To this end, preferably blocking agents containing groups that avoid nonspecific adsorption should be used. These blocking agents are typically ethanolamine and cysteine for protein coupling via amino groups and thiol groups, respectively. This report presents novel blocking agents containing poly(ethylene oxide) (PEO) groups. These blocking agents show enhanced qualities to avoid nonspecific adsorption and can therefore have advantages in versatile protein-surface technologies. (C) 2003 Elsevier B.V. All rights reserved.
引用
收藏
页码:67 / 74
页数:8
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