Coupling effects of osmotic pressure and temperature on the viability of Saccharomyces cerevisiae

被引:38
作者
Beney, L [1 ]
Marechal, PA [1 ]
Gervais, P [1 ]
机构
[1] Ecole Natl Super Biol Appl Nutr & Alimentat, Lab Genie Procedes Alimentaires & Biotechnol, F-21000 Dijon, France
关键词
D O I
10.1007/s002530100619
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The osmotic tolerance of cells of Saccharomyces cerevisiae as a function of glycerol concentration and temperature has been investigated. Results show that under isothermal conditions (25 degreesC) cells are resistant (94% viability) to hyperosmotic treatment at 49.2 MPa. A thigher osmotic pressure, cell viability decreases to 25% at 99 MPa. Yeast resistance to high osmotic stress (99 Mpa) is enhanced at low temperatures (5-41 degreesC). Therefore, the temperature at which hyperosmatic pressure is achieved greatly affects cell viability. These results suggest that temperature control is a suitable means of enhancing cell survival in response to osmotic dehydration.
引用
收藏
页码:513 / 516
页数:4
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