The tail structure of bacteriophage T4 and its mechanism of contraction

被引:154
作者
Kostyuchenko, VA
Chipman, PR
Leiman, PG
Arisaka, F
Mesyanzhinov, VV
Rossmann, MG
机构
[1] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA
[2] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia
[3] Tokyo Inst Technol, Grad Sch, Yokohama, Kanagawa 2268501, Japan
[4] Tokyo Inst Technol, Sch Biosci & Biotechnol, Yokohama, Kanagawa 2268501, Japan
基金
俄罗斯基础研究基金会; 美国国家科学基金会;
关键词
D O I
10.1038/nsmb975
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Bacteriophage T4 and related viruses have a contractile tail that serves as an efficient mechanical device for infecting bacteria. A three-dimensional cryo-EM reconstruction of the mature T4 tail assembly at 15-angstrom resolution shows the hexagonal dome-shaped baseplate, the extended contractile sheath, the long tail fibers attached to the baseplate and the collar formed by six whiskers that interact with the long tail fibers. Comparison with the structure of the contracted tail shows that tail contraction is associated with a substantial rearrangement of the domains within the sheath protein and results in shortening of the sheath to about one-third of its original length. During contraction, the tail tube extends beneath the baseplate by about one-half of its total length and rotates by 345 degrees, allowing it to cross the host's periplasmic space.
引用
收藏
页码:810 / 813
页数:4
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