Nonsteroidal anti-inflammatory drugs alter chloride and fluid transport in bovine retinal pigment epithelium

被引:22
作者
Bialek, S
Quong, JN
Yu, KF
Miller, SS
机构
[1] UNIV CALIF BERKELEY, SCH OPTOMETRY, BERKELEY, CA 94720 USA
[2] UNIV CALIF BERKELEY, DIV CELL & DEV, BERKELEY, CA 94720 USA
[3] UNIV CALIF BERKELEY, DEPT MOLEC & CELL BIOL, BERKELEY, CA 94720 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | 1996年 / 270卷 / 04期
关键词
fenamate; niflumic acid;
D O I
10.1152/ajpcell.1996.270.4.C1175
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Nonsteroidal anti-inflammatory drugs (NSAIDs) were added to the solutions bathing the apical membrane of bovine retinal pigment epithelium (RPE)-choroid explants. For example, niflumic acid (100 mu M) depolarized the basolateral membrane voltage (V-B) by similar to 12 mV, increased transepithelial potential by 4.5 mV, decreased intracellular Cl activity by 13 mM, decreased transepithelial resistance by 17 Omega . cm(2), and increased the ratio of apical to basolateral membrane resistance nearly threefold. All of these changes are consistent with an increase in basolateral membrane Cl conductance. In addition, niflumic acid caused intracellular Ca concentration to decrease by 16 nM and fluid transport rate to increase by 1.5 mu l . cm(-2) . h(-1). Flufenamic acid, which is structurally very similar to niflumic acid, had the opposite effects on membrane voltage and resistance. Basal application of the Cl channel blocker 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid or current clamping V-B to the reversal potential for Cl practically abolished the niflumic acid response. The niflumic acid response was unaffected by protein kinase inhibitors. These results suggest that certain NSAIDs can directly alter Cl conductance in the bovine RPE, apparently independently of cyclooxygenase inhibition.
引用
收藏
页码:C1175 / C1189
页数:15
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