Coffee diterpenes prevent benzo[a]pyrene genotoxicity in rat and human culture systems

被引：28

作者：

Cavin, C ^{[1
]}

Bezencon, C ^{[1
]}

Guignard, G ^{[1
]}

Schilter, B ^{[1
]}

机构：

[1] Nestle Res Ctr, Food Safety Grp, CH-1000 Lausanne 26, Switzerland

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2003年 / 306卷 / 02期

关键词：

cafestol and kahweol; coffee; diterpenes; benzo[a]pyrene; chemoprotection; blocking agent; metabolizing enzymes;

D O I：

10.1016/S0006-291X(03)00994-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The coffee-specific diterpenes cafestol and kahweol (C + K) have been identified as two important chemoprotective agents in coffee. In the present study, the potential preventive effects of C + K against the genotoxicity of B[a]P were investigated in rat primary hepatocytes and in human bronchial Beas-2B cells. Several independent mechanisms were identified and their respective contribution to the overall protective effects was determined. A marked dose-dependent inhibition by C + K of B[a]P DNA-binding was found in cells of both origins. However, data showed that the significant induction by C + K of the detoxifying enzyme GST-Yp subunit is the key mechanism of protection against B[a]P DNA-binding in rat liver, In contrast, the phase I-mediated mechanism where C + K produce an inhibition of CYP 1A1 induction by B[a]P is of key significance for the C + K protection in human Beas-2B cells. Moreover, this effect suggests a novel mechanism of chemoprotection by the coffee diterpenes cafestol and kahweol. (C) 2003 Elsevier Science (USA). All rights reserved.

引用

页码：488 / 495

页数：8

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