学术探索
学术期刊
新闻热点
数据分析
智能评审

Whole genome microarray analysis of C-elegans rrf-3 and eri-1 mutants

被引：15
作者：
Asikainen, Suvi
Storvik, Markus
Lakso, Meria
Wong, Garry
机构：
[1] Univ Kuopio, Dept Biosci, FIN-70211 Kuopio, Finland
[2] Univ Kuopio, AI Virtanen Inst, Dept Neurobiol, FIN-70211 Kuopio, Finland
来源：
FEBS LETTERS | 2007年 / 581卷 / 26期
关键词：
RNA interference; RRF-3; ERI-1; endogenous; RNAi; gene expression; c elegans;
D O I：
10.1016/j.febslet.2007.09.043
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We performed genome wide gene expression analysis on L4 stage Caenorhabditis elegans rrf-3(pk1426) and eri-1(mg366) mutant strains to study the effects caused by loss of their encoded proteins, which are required for the accumulation of endogenous secondary siRNAs. Mutant rrf-3 and eri-1 strains exhibited 72 transcripts that were co-over-expressed and 4 transcripts co-under-expressed (>2-fold, P < 0.05) compared to N2 wild type strain. Ontology analysis indicated these transcripts were over represented for protein phosphorylation and sperm function genes. These results provide additional support for the hypothesis that RRF-3 and ERI-1 act together in the endo-siRNA pathway, and furthermore suggests their involvement in additional biological processes. (C) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：5050 / 5054
页数：5
相关论文
共 20 条
[1]   MicroRNAs and other tiny endogenous RNAs in C-elegans [J].
Ambros, V ;
Lee, RC ;
Lavanway, A ;
Williams, PT ;
Jewell, D .
CURRENT BIOLOGY, 2003, 13 (10) :807-818
[2]  
BRENNER S, 1974, GENETICS, V77, P71
[3]   DAVID: Database for annotation, visualization, and integrated discovery [J].
Dennis, G ;
Sherman, BT ;
Hosack, DA ;
Yang, J ;
Gao, W ;
Lane, HC ;
Lempicki, RA .
GENOME BIOLOGY, 2003, 4 (09)
[4]  
Duchaine TF, 2006, CELL, V124, P343, DOI [10.1016/j.cell.2005.11.036, 10.1016/j.cel.2005.11.036]
[5]   Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans [J].
Fire, A ;
Xu, SQ ;
Montgomery, MK ;
Kostas, SA ;
Driver, SE ;
Mello, CC .
NATURE, 1998, 391 (6669) :806-811
[6]   A conserved siRNA-degrading RNase negatively regulates RNA interference in C-elegans [J].
Kennedy, S ;
Wang, D ;
Ruvkun, G .
NATURE, 2004, 427 (6975) :645-649
[7]   Interacting endogenous and exogenous RNAi pathways in Caenorhabditis elegans [J].
Lee, RC ;
Hammell, CM ;
Ambros, V .
RNA, 2006, 12 (04) :589-597
[8]   Animal virus replication and RNAi-mediated antiviral silencing in Caenorhabditis elegans [J].
Lu, R ;
Maduro, M ;
Li, F ;
Li, HW ;
Broitman-Maduro, G ;
Li, WX ;
Ding, SW .
NATURE, 2005, 436 (7053) :1040-1043
[9]   EGO-1, a putative RNA-dependent RNA polymerase, is required for heterochromatin assembly on unpaired DNA during C-elegans meiosis [J].
Maine, EM ;
Hauth, J ;
Ratliff, T ;
Vought, VE ;
She, XY ;
Kelly, WG .
CURRENT BIOLOGY, 2005, 15 (21) :1972-1978
[10]   Distinct populations of primary and secondary effectors during RNAi in C-elegans [J].
Pak, Julia ;
Fire, Andrew .
SCIENCE, 2007, 315 (5809) :241-244
12