Recent developments in the hammerhead ribozyme field

被引：64

作者：

Vaish, NK ^{[1
]}

Kore, AR ^{[1
]}

Eckstein, F ^{[1
]}

机构：

[1] Max Planck Inst Expt Med, D-37075 Gottingen, Germany

来源：

NUCLEIC ACIDS RESEARCH | 1998年 / 26卷 / 23期

关键词：

D O I：

10.1093/nar/26.23.5237

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Developments in the hammerhead ribozyme field during the last two years ape reviewed here. New results on the specificity of this ribozyme, the mechanism of its action and on the question of metal ion involvement in the-cleavage reaction are discussed. To demonstrate the potential of ribozyme technology examples of the application of this ribozyme for the inhibition of gene expression in cell culture, in animals, as well as in plant models are presented. Particular emphasis is given to critical steps in the approach, including RNA site selection, delivery, Vector development and cassette construction.

引用

页码：5237 / 5242

页数：6

共 79 条

[1]

ALI M, 1994, GENE THER, V1, P367

[2] The global conformation of an active hammerhead RNA during the process of self-cleavage [J].