Adding value to fusion proteins through covalent labelling

被引：70

作者：

Gronemeyer, T ^{[1
]}

Godin, G ^{[1
]}

Johnsson, K ^{[1
]}

机构：

[1] Ecole Polytech Fed Lausanne, Inst Chem Sci & Engn, CH-1015 Lausanne, Switzerland

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2005年 / 16卷 / 04期

关键词：

D O I：

10.1016/j.copbio.2005.06.001

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Recombinant proteins are often expressed with an additional polypeptide the role of which is to aid purification, detection or functional studies. Recently, the role of these tags has been extended to mediate the labelling of the protein of interest with chemically diverse compounds. These approaches are of importance for protein science as they allow proteins to be equipped with properties that cannot be genetically encoded and also enable the use of a single fusion protein for several different applications. Several new approaches have been developed for the covalent labelling of fusion proteins both in living cells and in vitro.

引用

页码：453 / 458

页数：6

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