Silver-enhanced colloidal gold electrochemical stripping detection of DNA hybridization

被引：264

作者：

Wang, J ^{[1
]}

Polsky, R ^{[1
]}

Xu, DK ^{[1
]}

机构：

[1] New Mexico State Univ, Dept Chem & Biochem, Las Cruces, NM 88003 USA

来源：

LANGMUIR | 2001年 / 17卷 / 19期

关键词：

D O I：

10.1021/la011002f

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report on a novel method for detecting DNA hybridization, based on the precipitation of silver on gold nanoparticle tags and a subsequent electrochemical stripping detection of the dissolved silver. Such coupling of a nanoparticle-promoted silver precipitation with the remarkable sensitivity of stripping metal analysis offers a dramatic enhancement of the hybridization response. An efficient magnetic isolation of the duplex is used for discriminating against nonhybridized DNA, including an excess of mismatched oligonucleotides. The new silver-enhanced colloidal gold stripping detection strategy holds great promise for the detection of DNA hybridization and represents an attractive alternative to indirect optical affinity assays of nucleic acids and other biomolecules.

引用

页码：5739 / 5741

页数：3

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