Extraction of Mycobacterium tuberculosis DNA:: a question of containment

被引:160
作者
Somerville, W
Thibert, L
Schwartzman, K
Behr, MA
机构
[1] McGill Univ, Hlth Ctr Res Inst, Montreal, PQ, Canada
[2] McGill Univ, Inst Natl Sante Publ Quebec, Dept Med, Montreal, PQ, Canada
[3] McGill Univ, Resp Epidemiol Unit, Montreal, PQ, Canada
关键词
D O I
10.1128/JCM.43.6.2996-2997.2005
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
DNA fingerprinting of Mycobacterium tuberculosis by IS6110 restriction fragment length polymorphism analysis requires substantial high-quality DNA. We demonstrated that, despite extraction treatments that might be expected to inactivate this organism, M. tuberculosis remained viable during this process. These data suggest that the extraction of M. tuberculosis DNA should be performed within containment until complete.
引用
收藏
页码:2996 / 2997
页数:2
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