Globin-coupled sensors: A class of heme-containing sensors in Archaea and Bacteria

被引:112
作者
Hou, SB
Freitas, T
Larsen, RW
Piatibratov, M
Sivozhelezov, V
Yamamoto, A
Meleshkevitch, EA
Zimmer, M
Ordal, GW
Alam, M [1 ]
机构
[1] Univ Hawaii, Dept Microbiol, Honolulu, HI 96822 USA
[2] Univ Hawaii, Dept Chem, Honolulu, HI 96822 USA
[3] Univ Florida, Whitney Lab, St Augustine, FL 32080 USA
[4] Univ Illinois, Dept Biochem, Urbana, IL 61801 USA
[5] Marine Bioprod Engn Ctr, Honolulu, HI 96822 USA
关键词
proximal histidine; transducer;
D O I
10.1073/pnas.161185598
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The recently discovered prokaryotic signal transducer HemAT, which has been described in both Archaea and Bacteria, mediates aerotactic responses. The N-terminal regions of HemAT from the archaeon Halobacterium salinarum (HemAT-Hs) and from the Gram-positive bacterium Bacillus subtilis (HemAT-Bs) contain a myoglobin-like motif, display characteristic heme-protein absorption spectra, and bind oxygen reversibly. Recombinant HemAT-Hs and HemAT-Bs shorter than 195 and 176 residues, respectively, do not bind heme effectively. Sequence homology comparisons and three-dimensional modeling predict that His-123 is the proximal heme-binding residue in HemAT from both species. The work described here used site-specific mutagenesis and spectroscopy to confirm this prediction, thereby providing direct evidence for a functional domain of prokaryotic signal transducers that bind heme in a globin fold. We postulate that this domain is part of a globin-coupled sensor (GCS) motif that exists as a two-domain transducer having no similarity to the PER-ARNT-SIM (PAS)-domain superfamily transducers. Using the GCS motif, we have identified several two-domain sensors in a variety of prokaryotes. We have cloned, expressed, and purified two potential globin-coupled sensors and performed spectral analysis on them. Both bind heme and show myoglobin-like spectra. This observation suggests that the general function of GCS-type transducers is to bind diatomic oxygen and perhaps other gaseous ligands, and to transmit a conformational signal through a linked signaling domain.
引用
收藏
页码:9353 / 9358
页数:6
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