Delivery of membrane proteins into small and giant unilamellar vesicles by charge-mediated fusion

被引：47

作者：

Biner, Olivier ^{[1
,2
]}

Schick, Thomas ^{[1
,2
]}

Muller, Yannic ^{[1
]}

von Ballmoos, Christoph ^{[1
]}

机构：

[1] Univ Bern, Dept Chem & Biochem, Freiestr 3, CH-3012 Bern, Switzerland

[2] Univ Bern, Grad Sch Cellular & Biomed Sci, CH-3012 Bern, Switzerland

来源：

FEBS LETTERS | 2016年 / 590卷 / 14期

基金：

瑞士国家科学基金会;

关键词：

charged lipids; giant unilamellar vesicle; liposomes; membrane fusion; protein reconstitution; respiratory chain enzymes; ARTIFICIAL CELL; LIPID VESICLES; LIPOSOMES; RECONSTITUTION; TRANSPORT;

D O I：

10.1002/1873-3468.12233

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

One of the current challenges in synthetic biology is the production of stable membrane mimetic systems and the insertion of components in these systems. Here, we employ fusion of oppositely charged liposomes to deliver separately reconstituted membrane proteins into a common lipid bilayer. After a systematic evaluation of different lipid compositions by lipid mixing and size distribution analysis, suitable conditions were further investigated for proteoliposome fusion. With this technique, we functionally coreconstituted bo(3) oxidase and ATP synthase from Escherichia coli into unilamellar liposomes ranging from 100 nm to 50 mu m in size. The presented method is a simple and versatile tool for oriented membrane protein reconstitution to produce biomimetic systems with increased complexity.

引用

页码：2051 / 2062

页数：12

共 38 条

[1]

Functional Reconstitution of a Voltage-Gated Potassium Channel in Giant Unilamellar Vesicles [J].