Cytoskeletal remodelling and slow dynamics in the living cell

被引：387

作者：

Bursac, P

Lenormand, G

Fabry, B

Oliver, M

Weitz, DA

Viasnoff, V

Butler, JP

Fredberg, JJ

机构：

[1] Harvard Univ, Sch Publ Hlth, Physiol Program, Boston, MA 02115 USA

[2] Univ Erlangen Nurnberg, Inst Biomed Technol, D-91054 Erlangen, Germany

[3] Harvard Univ, Div Engn & Appl Sci, Cambridge, MA 02138 USA

[4] Harvard Univ, Rowland Inst Sci, Cambridge, MA 02142 USA

[5] Tohoku Univ, Sch Med, Dept Geriatr & Resp Med, Sendai, Miyagi 9808574, Japan

来源：

NATURE MATERIALS | 2005年 / 4卷 / 07期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1038/nmat1404

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Forced and spontaneous motions of microbeads tightly bound to the cytoskeleton (CSK) of human muscle cells were analyzed. HASM cells in passages 6-7 were serum-deprived for 24 h; serum deprivation arrests the cell cylce in the G1/G00 phases. It was observed that these spontaneous bead motions reflecting nanoscale CSK arrangements depended on both the approach to glass transition and energy release due to ATP hydrolysis. The results show that the trapping, intermittency, and approach to kinetic arrest are mesoscale features of CSK protein-protein interaction that link integrative phenotypic functions to the molecular events.

引用

页码：557 / 561

页数：5

共 33 条

[1] Microrheology of human lung epithelial cells measured by atomic force microscopy
Alcaraz, J
Buscemi, L
Grabulosa, M
Trepat, X
Fabry, B
Farré, R
Navajas, D
[J]. BIOPHYSICAL JOURNAL, 2003, 84 (03) : 2071 - 2079
[2] Role of heat shock protein 27 in cytoskeletal remodeling of the airway smooth muscle cell
An, SS
Fabry, B
Mellema, M
Bursac, P
Gerthoffer, WT
Kayyali, US
Gaestel, M
Shore, SA
Fredberg, JJ
[J]. JOURNAL OF APPLIED PHYSIOLOGY, 2004, 96 (05) : 1701 - 1713
[3] Stiffness changes in cultured airway smooth muscle cells
An, SS
Laudadio, RE
Lai, J
Rogers, RA
Fredberg, JJ
[J]. AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2002, 283 (03): : C792 - C801
[4] Intermittent dynamics and hyper-aging in dense colloidal gels
Bissig, H
Romer, S
Cipelletti, L
Trappe, V
Schurtenberger, P
[J]. PHYSCHEMCOMM, 2003, 6 : 21 - 23
[5] Universal non-diffusive slow dynamics in aging soft matter
Cipelletti, L
Ramos, L
Manley, S
Pitard, E
Weitz, DA
Pashkovski, EE
Johansson, M
[J]. FARADAY DISCUSSIONS, 2003, 123 : 237 - 251
[6] Rheological aging and rejuvenation in microgel pastes
Cloitre, M
Borrega, R
Leibler, L
[J]. PHYSICAL REVIEW LETTERS, 2000, 85 (22) : 4819 - 4822
[7] Reverse engineering of biological complexity
Csete, ME
Doyle, JC
[J]. SCIENCE, 2002, 295 (5560) : 1664 - 1669
[8] Energy flow, partial equilibration, and effective temperatures in systems with slow dynamics
Cugliandolo, LF
Kurchan, J
Peliti, L
[J]. PHYSICAL REVIEW E, 1997, 55 (04): : 3898 - 3914
[9] Aging and nonlinear rheology in suspensions of polyethylene oxide-protected silica particles
Derec, C
Ducouret, G
Ajdari, A
Lequeux, F
[J]. PHYSICAL REVIEW E, 2003, 67 (06): : 9 - 061403
[10] Creep function of a single living cell
Desprat, N
Richert, A
Simeon, J
Asnacios, A
[J]. BIOPHYSICAL JOURNAL, 2005, 88 (03) : 2224 - 2233

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