Ubiquitin dependent proteolysis is activated in apoptotic fibroblasts in culture

被引：11

作者：

Bresin, A

Iacoangeli, A

Risuleo, G

Scarsella, G

机构：

[1] Univ Rome La Sapienza, Dipartimento Genet & Biol Mol, I-00185 Rome, Italy

[2] Univ Rome La Sapienza, Dipartimento Biol Cellulare & Sviluppo, I-00185 Rome, Italy

来源：

MOLECULAR AND CELLULAR BIOCHEMISTRY | 2001年 / 220卷 / 1-2期

关键词：

cell stress; ubiquitin; proteasome; apoptosis;

D O I：

10.1023/A:1010862021958

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The ubiquitin mediated pathway constitutes an early response in cultured cells where apoptosis, assessed by internucleosomal specific DNA fragmentation, was induced by serum withdrawal. Data demonstrate that nuclear ubiquitin proteolytic system, but not cytoplasmic, is activated. This activation is paralleled by a substantial chromatin de-condensation. We suggest that chromatin relaxation is causative of the fragmentation since it exposes the DNA to nucleolytic attack. Finally, maintenance of homeostasis and induction of apoptosis seem to undergo a parallel contemporary pathway with a possible mutual feedback.

引用

页码：57 / 60

页数：4

共 20 条

[1] Oxidative damage, bleomycin, and gamma radiation induce different types of DNA strand breaks in normal lymphocytes and thymocytes -: A comet assay study [J].