High-performance liquid chromatographic enantioseparation of (R,S)-fluoxetine using Marfey's reagent and (S)-N-(4-nitrophenoxycarbonyl) phenylalanine methoxyethyl ester as chiral derivatizing reagents along with direct thin-layer chromatographic resolution and isolation of enantiomers using L-tartaric acid as mobile phase additive

Chiral assay of enantiomers of fluoxetine was achieved in pharmaceutical formulations using direct and indirect methods L-Tartaric acid was used as a mobile phase additive in thin-layer chromatography, the enantiomers were separated and isolated and were used to determine the elution order in HPLC (R,S)-flouxetine was derivatized with (S)-N-(4-nitrophenoxycarbonyl)phenylalanine methoxyethyl ester [(S)-NIFE] Marfey's reagent and 1-fluoro-2,4-dinitrophenyl-L-methionine amide (FDNP-L-Met-NH2) The diastereomers were separated using RP-HPLC The effect of flow rate and TFA concentration on resolution was studied The diastereomers obtained by derivatization with FDNP-L-Met-NH2 were also separated by RP-TLC Copyright (C) 2010 John Wiley & Sons, Ltd