Adhesion of adenosine diphosphate-activated platelets to human brain microvascular endothelial cells under flow in vitro is mediated via GPIIb/IIIa

被引：7

作者：

Tanahashi, N ^{[1
]}

Fukuuchi, Y ^{[1
]}

Tomita, M ^{[1
]}

Tomita, Y ^{[1
]}

Inoue, K ^{[1
]}

Satoh, H ^{[1
]}

Abe, T ^{[1
]}

机构：

[1] Sch Med, Dept Neurol, Shinjuku Ku, Tokyo 1608582, Japan

来源：

NEUROSCIENCE LETTERS | 2001年 / 301卷 / 01期

关键词：

platelet adhesion; human brain microvascular endothelial cells; GPIIb/IIIa; GPIb alpha; platelet aggregation;

D O I：

10.1016/S0304-3940(01)01608-1

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Employing video-enhanced contrast (VEC) microscopy, we examined whether TAK-029 (GPIIb/IIIa antagonist) inhibits the adhesion of activated platelets to human brain microvascular endothelial cells (HBEC) in vitro. HBECs were cultured on a coverglass and put in the observation chamber of VEC microscopy. Then, activated platelets by adenosine diphosphate (ADP) (2 muM) were perfused over HBEC at a low shear rate of 10 s(-1) for 30 min and washed out. Platelets adhered directly to HBEC. However, platelet adhesion to HBEC was suppressed when platelet rich plasma with ADP (2 muM) plus TAK-029 (GPIIb/IIIa antagonist; 1 muM) was perfused over HBEC for 30 min and washed out. Anti-GPIb alpha. antibody (GUR20-5) did not inhibit adhesion of ADP-activated platelets to HBEC. The above results showed adhesion of ADP-activated platelets to HBEC under flow in vitro is mediated via GPIIb/IIIa (C) 2001 Published by Elsevier Science Ireland Ltd.

引用

页码：33 / 36

页数：4

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