Molecular cloning of full-length sequence of hepatitis G virus genome isolated from a Japanese patient with liver disease

被引：6

作者：

Kaneko, T

Hayashi, S

Arakawa, Y

Abe, K

机构：

[1] Natl Inst Infect Dis, Dept Pathol, Shinjuku Ku, Tokyo, Japan

[2] Nihon Univ, Sch Med, Dept Internal Med 3, Tokyo, Japan

[3] Int Med Ctr Japan, Div Gastroenterol, Tokyo, Japan

来源：

HEPATOLOGY RESEARCH | 1998年 / 12卷 / 03期

关键词：

Japanese HGV isolate; full-length sequence of HGV genome; HGV genotypes;

D O I：

10.1016/S1386-6346(98)00064-3

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

A transfusion-transmissible agent, designated hepatitis G virus (HGV), was recently identified. We have cloned the full-length nucleotide sequence of the HGV genome (denoted HGV-IM71) recovered from a Japanese patient with liver cirrhosis. The HGV-IM71 isolate was composed of 9387 nucleotides (nt) with 5' and 3' untranslated regions of 458 and 310 nt, respectively. It had a single open reading frame, encoding 2873 amino acid (aa) residues. This isolate differed from previously reported HGV/GBV-C isolates by 8-15% of the nucleotide sequence and 3-5% of the amino acid sequence. The isolate lacked a clearly identifiable core gene that had only 47 aa residues. Based on phylogenetic analysis of the full-genome sequence, it was concluded that HGV-IM71 belonged to the Asia type (type 3) of HGV genotypes. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：207 / 216

页数：10

共 34 条

[1] Prevalence of hepatitis G virus infection among patients with liver diseases in Japan [J].