Contrasting patterns of regulation of the antioxidant selenoproteins, thioredoxin reductase, and glutathione peroxidase, in cancer cells

被引:107
作者
Gladyshev, VN [1 ]
Factor, VM
Housseau, F
Hatfield, DL
机构
[1] Univ Nebraska, Dept Biochem, Lincoln, NE 68588 USA
[2] NCI, Basic Res Labs, NIH, Bethesda, MD 20892 USA
[3] NCI, Expt Carcinogenesis Lab, NIH, Bethesda, MD 20892 USA
[4] NCI, Surg Branch, NIH, Bethesda, MD 20892 USA
基金
美国国家卫生研究院;
关键词
D O I
10.1006/bbrc.1998.9495
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
There is strong evidence that selenium protects against certain human cancers, but the underlying mechanism is unknown. Glutathione peroxidase (GPX1) and thioredoxin reductase (TR), the most abundant antioxidant selenium-containing proteins in mammals, have been implicated in this protection. me analyzed the expression of TR and GPX1 in the following model cancer systems: (1) liver tumors in TGF alpha/c-myc transgenic mice; (2) human prostate cell lines from normal and cancer tissues; and (3) p53-induced apoptosis in a human colon cancer cell line. TR was induced while GPX1 was repressed in malignancies relative to controls in transgenic mice and prostate cell Lines. In the colon cell line, p53 expression resulted in elevated GPX1, but repressed TR. The data indicate that TR and GPX1 are regulated in a contrasting manner in the cancer systems tested and reveal the p53-dependent regulation of selenoprotein expression. The data suggest that additional studies on selenoprotein regulation in different cancers are required to evaluate future implementation of selenium as a dietary supplement in individuals at risk for developing certain cancers. (C) 1998 Academic Press.
引用
收藏
页码:488 / 493
页数:6
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