The solution NMR structure of a blue-green algae hepatotoxin, microcystin-RR - A comparison with the structure of microcystin-LR

被引:14
作者
Trogen, GB
Edlund, U
Larsson, G
Sethson, I [1 ]
机构
[1] Umea Univ, Dept Organ Chem, S-90187 Umea, Sweden
[2] Umea Univ, Dept Med Biochem & Biophys, S-90187 Umea, Sweden
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1998年 / 258卷 / 02期
关键词
microcystins; cyanobacteria; protein phosphatase; three-dimensional structure; simulated annealing;
D O I
10.1046/j.1432-1327.1998.2580301.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The microcystin-RR structures are compared with the structures of microcystin-LR in solution as well as in the crystal structure of the complex with protein phosphatase. The gross structures of the two peptides are similar, but with a more accentuated and compact saddle structure for microcystin-RR. The structural differences affect the hydrogen-bond pattern in the peptides and the location of the side chain of N-methyldehydroalanine, both of which are important for the ability of the peptide to form a tight complex with protein phosphatase. These structural differences may contribute to the observed differences in toxicity of microcystin-RR and microcystin-LR.
引用
收藏
页码:301 / 312
页数:12
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