A novel transporter involved in cobalt uptake

被引:96
作者
Komeda, H [1 ]
Kobayashi, M [1 ]
Shimizu, S [1 ]
机构
[1] KYOTO UNIV,FAC AGR,DEPT AGR CHEM,SAKYO KU,KYOTO 60601,JAPAN
关键词
transport; metal; nitrile hydratase; membrane protein; nickel;
D O I
10.1073/pnas.94.1.36
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Cobalt is an essential component of a low molecular-mass nitrile hydratase (L-NHase) from Rhodococcus rhodochrous J1. We have found a new gene, nhlF, in the DNA region sandwiched between nhlBA encoding L-NHase and amdA encoding amidase, which are involved in the degradation of nitriles, The product of nhlF, NhlF, shows a significant sequence similarity with those of hoxN from Alcaligenes eutrophus, hupN from Bradyrhizobium japonicum, nixA from Helicobacter pylori, and ureH from Bacillus sp,, which are considered to be involved in nickel uptake into these cells, Sequence and hydropathy plot analyses have shown that NhlF encodes a 352-amino acid (aa) protein with eight hydrophobic putative membrane-spanning domains. nhlF expression in R. rhodochrous ATCC 12674 and Escherichia coli JM109 confers uptake of Co-57 in their cells, but not of Ni-63, The expression of both nhlF and nhlBA in R. rhodochrous ATCC 12674 exhibited higher NHase activity than nhlBA expression. These findings together with the inhibitory effect by uncouplers (CCCP end SP6847) for the cobalt uptake suggest that NhlF mediates the cobalt transport into the cell energy-dependently finally to provide L-NHase.
引用
收藏
页码:36 / 41
页数:6
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