Regulation of endosome fusion

被引:50
作者
Mills, IG [1 ]
Jones, AT [1 ]
Clague, MJ [1 ]
机构
[1] Univ Liverpool, Physiol Lab, Liverpool L69 3BX, Merseyside, England
基金
英国惠康基金;
关键词
endosome fusion; rab5; early endosomal autoantigen 1; phosphatidylinositol; 3-kinase;
D O I
10.1080/096876899294788
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Homotypic fusion between early endosomes can be reconstituted in vitro. By using wortmannin and LY294002, inhibitors of phosphatidylinositol (PI) 3-kinase, a requirement for this activity has been established in order for fusion to proceed efficiently. It has been shown that PI 3-kinase activity is required downstream of rab5 activation, although a large excess of activated rab5 can overcome wortmannin inhibition. A series of experiments have also been performed which indicate a role for early endosomal autoantigen 1 (EEA1) in determining fusion efficiency, EEA1 dissociates from membranes following wortmannin treatment. It is proposed that the requirement of endosome fusion for PI 3-kinase activity is to promote the association of EEA1 with endosomes.
引用
收藏
页码:73 / 79
页数:7
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