Placement of an Elastic Biodegradable Cardiac Patch on a Subacute Infarcted Heart Leads to Cellularization With Early Developmental Cardiomyocyte Characteristics

被引:44
作者
Fujimoto, Kazuro L. [1 ,2 ]
Tobita, Kimimasa [1 ,3 ,4 ,5 ]
Guan, Jianjun [1 ,6 ]
Hashizume, Ryotaro [1 ,2 ]
Takanari, Keisuke [1 ,2 ]
Alfieri, Christina M. [7 ]
Yutzey, Katherine E. [7 ]
Wagner, William R. [1 ,2 ,4 ,5 ]
机构
[1] Univ Pittsburgh, McGowan Inst Regenerat Med, Pittsburgh, PA 15219 USA
[2] Univ Pittsburgh, Dept Surg, Pittsburgh, PA 15219 USA
[3] Univ Pittsburgh, Dept Dev Biol, Pittsburgh, PA 15219 USA
[4] Univ Pittsburgh, Dept Bioengn, Pittsburgh, PA 15219 USA
[5] Univ Pittsburgh, Dept Chem Engn, Pittsburgh, PA 15219 USA
[6] Ohio State Univ, Dept Mat Sci & Engn, Columbus, OH 43210 USA
[7] Cincinnati Childrens Med Ctr, Div Mol Cardiovasc Biol, Cincinnati, OH USA
关键词
Infarction; remodeling; patch implantation; cellularization; SMOOTH-MUSCLE ACTIN; STEM-CELLS; MYOCARDIAL-INFARCTION; GENE-EXPRESSION; REPAIR; REGENERATION; PATTERNS; MYOCYTES; ISOFORM; NKX-2.5;
D O I
10.1016/j.cardfail.2012.05.006
中图分类号
R5 [内科学];
学科分类号
100201 [内科学];
摘要
Background: Placement of an elastic biodegradable patch onto a subacute myocardial infarct (MI) provides temporary elastic support that may act to effectively alter adverse left ventricular (LV) remodeling processes. Methods: Two weeks after permanent left coronary ligation in Lewis rats, the infarcted anterior wall was covered with polyester urethane urea (MI + PEUU; n = 15) or expanded polytetratluoroethylene (MI + ePTFE; n = 15) patches, or had no implantation (MI + sham; n = 12). Eight weeks after surgery, cardiac function and histology were assessed. Results: The ventricular wall in the MI + ePTFE and MI + sham groups was composed of fibrous tissue, whereas PEUU implantation induced alpha-smooth muscle actin positive muscle bundles coexpressing sarcomeric alpha-actinin and cardiac-specific troponin-T. This pattern of colocalization was also found in developing embryonic myocardium. Cardiac transcription factors Nkx-2.5 and GATA-4 were strongly expressed in the muscle bundles. In the MI + sham group, end-diastolic LV cavity area (EDA) increased and the percentage of fractional area change (%FAC) decreased. For ePTEE patched animals, both EDA and %FAC decreased. In contrast, with MI + PEUU patching, %FAC increased and EDA was maintained. With dobutamine-stress echocardiography, MI + PEUU patched LVs possessed contractile reserve significantly larger than the MI + sham group. Conclusions: MI + PEUU patch implantation onto subacute infarcted myocardium induced muscle cellularization with characteristics of early developmental cardiomyocytes as well as providing a functional reserve. (J Cardiac Fail 2012:18:585-595)
引用
收藏
页码:585 / 595
页数:11
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