A nonviral transfection approach in vitro: The design of a gold nanoparticle vector joint with microelectromechanical systems

被引:46
作者
Jen, CP
Chen, YH
Fan, CS
Yeh, CS [1 ]
Lin, YC
Shieh, DB
Wu, CL
Chen, DH
Chou, CH
机构
[1] Natl Cheng Kung Univ, Dept Chem, Tainan 701, Taiwan
[2] Natl Cheng Kung Univ, Dept Engn Sci, Tainan 701, Taiwan
[3] Natl Cheng Kung Univ, Dept Dent, Tainan 701, Taiwan
[4] Natl Cheng Kung Univ, Dept Biochem, Tainan 701, Taiwan
[5] Natl Cheng Kung Univ, Dept Chem Engn, Tainan 701, Taiwan
[6] Natl Cheng Kung Univ, Dept Clin Pharm, Tainan 701, Taiwan
关键词
D O I
10.1021/la036154k
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
An nanoparticles modified with 21-base thiolated-oligonucleotides have been evaluated as delivery vehicles for the development of a nonviral transfection platform. The electromigration combined with electroporation for DNA delivery in an osteoblast like cell was employed to test on microchips. Electroporation introduces foreign materials into cells by applying impulses of electric field to induce multiple transient pores on the cell membrane through dielectric breakdown of the cell membrane. On the basis of the characteristic surface plasmon of the An particles, UV-vis absorption was utilized to qualitatively judge the efficiency of delivery. Transmission electron microscopy images and atomic absorption measurements (quantitative analysis) provided evidence of the bare An and Au/oligonucleotide nanoparticles before and after electroporation and electromigration function. The experiments demonstrated that electrophoretic migration followed by electroporation significantly enhanced the transportation efficiency of the nanoparticle-oligonucleotide complexes as compared with electroporation alone. Most interestingly, An capped with oligonucleotides led to optimal performance. On the other hand, the bare Au colloidal suspensions resulted in aggregation, which might be an obstacle to the internalization process. In addition, analytical results demonstrated an increase in the local particle concentrations on the cell surface that provided additional support for the mechanism underlying the improved Au nanoparticle transportation into cells in the presence of electromigration function.
引用
收藏
页码:1369 / 1374
页数:6
相关论文
共 18 条
  • [1] Bird R.B., 2006, TRANSPORT PHENOMENA, Vsecond, DOI 10.1002/aic.690070245
  • [2] Budker V, 2000, J GENE MED, V2, P76, DOI 10.1002/(SICI)1521-2254(200003/04)2:2<76::AID-JGM97>3.0.CO
  • [3] 2-4
  • [4] Dennig Jorg, 2002, J Biotechnol, V90, P339
  • [5] CATIONIC LIPOSOME-MEDIATED TRANSFECTION
    FELGNER, PL
    RINGOLD, GM
    [J]. NATURE, 1989, 337 (6205) : 387 - 388
  • [6] PREPARATION AND CHARACTERIZATION OF AU COLLOID MONOLAYERS
    GRABAR, KC
    FREEMAN, RG
    HOMMER, MB
    NATAN, MJ
    [J]. ANALYTICAL CHEMISTRY, 1995, 67 (04) : 735 - 743
  • [7] HAN R, 2002, VACCINE, V18, P2973
  • [8] HOFMANN GA, 1986, IEEE ENG MED BIOL, V12, P6
  • [9] A nonviral DNA delivery system based on surface modified silica-nanoparticles can efficiently transfect cells in vitro
    Kneuer, C
    Sameti, M
    Bakowsky, U
    Schiestel, T
    Schirra, H
    Schmidt, H
    Lehr, CM
    [J]. BIOCONJUGATE CHEMISTRY, 2000, 11 (06) : 926 - 932
  • [10] Electroporation microchips for in vitro gene transfection
    Lin, YC
    Huang, MY
    [J]. JOURNAL OF MICROMECHANICS AND MICROENGINEERING, 2001, 11 (05) : 542 - 547