Direct involvement of hydrogen peroxide in bacterial alpha-hydroxylation of fatty acid

被引：46

作者：

Matsunaga, I ^{[1
]}

Yamada, M ^{[1
]}

Kusunose, E ^{[1
]}

Nishiuchi, Y ^{[1
]}

Yano, I ^{[1
]}

Ichihara, K ^{[1
]}

机构：

[1] OSAKA CITY UNIV, SCH MED, DEPT BACTERIOL, OSAKA 545, JAPAN

来源：

FEBS LETTERS | 1996年 / 386卷 / 2-3期

关键词：

fatty acid; alpha-oxidation; hydrogen peroxide; Sphingomonas paucimobilis;

D O I：

10.1016/0014-5793(96)00451-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have reported that fatty-acid alpha-hydroxylase partially purified from Sphingomonas paucimobilis required NADH and molecular oxygen. In this study, we found that the reaction was greatly inhibited by catalase. Glutathione and glutathione peroxidase also inhibited alpha-hydroxylation, but superoxide dismutase and mannitol did not. Replacement of NADH and molecular oxygen by hydrogen peroxide increased the alpha-hydroxylation activity. In the presence of hydrogen peroxide, molecular oxygen was not required for the activity. These findings suggest that hydrogen peroxide was essential for bacterial alpha-hydroxylase.

引用

页码：252 / 254

页数：3

共 15 条

[1]

ANDERSSON KK, 1991, NEW J CHEM, V15, P411

[2]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[3] MECHANISM OF ALPHA-OXIDATION IN LEAVES [J].