Molecular typing of clinical and environmental isolates of Scedosporium prolificans by inter-simple-sequence-repeat polymerase chain reaction

被引:17
作者
Solé, M
Cano, J
Rodríguez-Tudela, JL
Pontón, J
Sutton, DA
Perrie, R
Gené, J
Rodríguez, V
Guarro, J
机构
[1] Univ Rovira & Virgili, Fac Med & Ciencies Salut, Unitat Microbiol, Inst Estudis Avancats, Tarragona 43201, Spain
[2] Ctr Nacl Microbiol, Unidad Micol, Majadahonda, Spain
[3] Univ Basque Country, Dept Microbiol, E-48080 Bilbao, Spain
[4] Univ Texas, Hlth Sci Ctr, Dept Pathol, San Antonio, TX 78284 USA
[5] Womens & Childrens Hosp, Mycol Unit, Adelaide, SA, Australia
[6] Hosp Valle De Hebron, Serv Microbiol & Parasitol, Barcelona, Spain
关键词
emerging fungi; ISSR-PCR; molecular epidemiology; Scedosporium prolificans; AMPLIFIED MICROSATELLITES RAMS; PCR AMPLIFICATION; GENETIC-VARIATION; RDNA SEQUENCES; FUNGI; INFLATUM; PSEUDALLESCHERIA; DIFFERENTIATION; ENDOPHYTES; PATHOGEN;
D O I
10.1080/13693780310001600813
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Invasive infections by Scedosporium prolificans have increased alarmingly in recent years, mainly in immunosuppressed patients. The epidemiology, pathogenesis and the natural habitat of this pathogen are practically unknown. Isolates of S. prolificans were distinguished from one another by inter-simple-sequence-repeat (ISSR) fingerprinting, a technique based on the high degree of polymorphism of the multisatellite genetic markers used. This technique was found useful for typing 84 isolates of S. prolificans from different countries and sources. The assemblage of S. prolificans isolates tested was extremely diverse, with 35 genotypes present. Several patients were found to have been infected or colonized by more than one strain. Overall, this technique facilitates the epidemiological study of S. prolificans infection.
引用
收藏
页码:293 / 300
页数:8
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