Cell damage in near-infrared multimode optical traps as a result of multiphoton absorption

被引：128

作者：

Konig, K ^{[1
]}

Liang, H ^{[1
]}

Berns, MW ^{[1
]}

Tromberg, BJ ^{[1
]}

机构：

[1] UNIV CALIF IRVINE, BECKMAN LASER INST & MED CLIN, IRVINE, CA 92715 USA

来源：

OPTICS LETTERS | 1996年 / 21卷 / 14期

关键词：

D O I：

10.1364/OL.21.001090

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We report on cell damage of single cells confined in continuous-wave (cw), near-infrared (NIR) multimode optical traps as a result of multiphoton absorption phenomena. Trapping beams at NIR wavelengths less than 800 nm are capable of damaging cells through a two-photon absorption process. Cell damage is more pronounced in multimode cw traps compared with single-frequency true cw NIR traps because of transient power enhancement by longitudinal mode beating. Partial mode locking in tunable cw Ti:sapphire lasers used as trapping beam sources can produce unstable subnanosecond pulses at certain wavelengths that amplify multiphoton absorption effects significantly. We recommend the use of single-frequency long-wavelength NIR trapping beams for optical micromanipulation of vital cells. (C) 1996 Optical Society of America

引用

页码：1090 / 1092

页数：3

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