Heliquinomycin, a new inhibitor of DNA helicase, produced by Streptomyces sp MJ929-SF2 .1. Taxonomy, production, isolation, physico-chemical properties and biological activities

被引：59

作者：

Chino, M ^{[1
]}

Nishikawa, K ^{[1
]}

Umekita, M ^{[1
]}

Hayashi, C ^{[1
]}

Yamazaki, T ^{[1
]}

Tsuchida, T ^{[1
]}

Sawa, T ^{[1
]}

Hamada, M ^{[1
]}

Takeuchi, T ^{[1
]}

机构：

[1] NIPPON KAYAKU CO LTD,PHARMACEUT GRP,KITA KU,TOKYO 115,JAPAN

来源：

JOURNAL OF ANTIBIOTICS | 1996年 / 49卷 / 08期

关键词：

D O I：

10.7164/antibiotics.49.752

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Heliquinomycin was isolated as a part of a program designed to find inhibitors of DNA helicase from microbial sources. It was purified from the culture broth of Streptomyces sp. MJ929-SF2 by solvent extraction and serial chromatographies of centrifugal partition chromatography, Sephadex LH-20 and Capcell Pak C18 (HPLC). The isolated red powder was analyzed to have the molecular formula of C33H30O17. It inhibited partially purified DNA helicase from HeLa cell in a noncompetitive manner with the inhibition constant (Ki) of 6.8 mM. Heliquinomycin exhibited biological activity against microorganisms including MRSA, and cultured cell lines.

引用

页码：752 / 757

页数：6

共 16 条

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