Identification of a γ-interferon-responsive element in the promoter of the human macrophage scavenger receptor A gene

被引：34

作者：

Grewal, T ^{[1
]}

Priceputu, E ^{[1
]}

Davignon, J ^{[1
]}

Bernier, L ^{[1
]}

机构：

[1] Clin Res Inst Montreal, Hyperlipidemia & Atherosclerosis Res Grp, Montreal, PQ H2W 1R7, Canada

来源：

ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY | 2001年 / 21卷 / 05期

关键词：

scavenger receptor type A; gamma-interferon; STAT1; alpha; GAS element; macrophage activation;

D O I：

10.1161/01.ATV.21.5.825

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

In the present study, we demonstrate gamma -interferon (gamma -IFN)-inducible scavenger receptor A (SR-A) mRNA expression during the early stages of THP-1 and blood monocyte differentiation. Predominant induction of SR-A type II mRNA parallels the increased accumulation of cholesteryl esters under these conditions. A potential signal transducer and activator of transcription (STAT1) binding site (gamma -interferon activation site) in the SR-A promoter demonstrates gamma -IFN-inducible DNA binding activity and is most likely responsible for the gamma -IFN-dependent expression of an SR-A promoter-luciferase fusion construct. In contrast, gamma -IFN inhibits SR-A expression in mature macrophages as well as after prolonged gamma -IFN incubation of THP-1 monocytes. Taken together, these results demonstrate opposite effects of gamma -IFN on SR-A expression and activity during the early versus late stages of monocyte maturation. gamma -IFN-induced STAT1 activation, leading to increased SR-A expression, could therefore play an important role in the initial steps of foam cell formation and xanthomatosis.

引用

页码：825 / 831

页数：7

共 29 条

[1]

ASHKENAS J, 1993, J LIPID RES, V34, P983

[2]

BOTTALICO LA, 1991, J BIOL CHEM, V266, P22866

[3] STAT1 activation during monocyte to macrophage maturation: role of adhesion molecules [J].

Coccia, EM ;

Del Russo, N ;

Stellacci, E ;

Testa, U ;

Marziali, G ;

Battistini, A .

INTERNATIONAL IMMUNOLOGY, 1999, 11 (07) :1075-1083

[4] MACROPHAGE-COLONY-STIMULATING FACTOR SELECTIVELY ENHANCES MACROPHAGE SCAVENGER RECEPTOR EXPRESSION AND FUNCTION [J].

DEVILLIERS, WJS ;

FRASER, IP ;

HUGHES, DA ;

DOYLE, AG ;

GORDON, S .

JOURNAL OF EXPERIMENTAL MEDICINE, 1994, 180 (02) :705-709

[5]

EMI M, 1993, J BIOL CHEM, V268, P2120

[6] DIVALENT CATION-INDEPENDENT MACROPHAGE ADHESION INHIBITED BY MONOCLONAL-ANTIBODY TO MURINE SCAVENGER RECEPTOR [J].

FRASER, I ;

HUGHES, D ;

GORDON, S .

NATURE, 1993, 364 (6435) :343-345

[7] DIFFERENTIAL EXPRESSION OF SCAVENGER RECEPTOR ISOFORMS DURING MONOCYTE-MACROPHAGE DIFFERENTIATION AND FOAM CELL-FORMATION [J].

GENG, YJ ;

KODAMA, T ;

HANSSON, GK .

ARTERIOSCLEROSIS AND THROMBOSIS, 1994, 14 (05) :798-806

[8] INTERFERON-GAMMA INHIBITS SCAVENGER RECEPTOR EXPRESSION AND FOAM CELL-FORMATION IN HUMAN MONOCYTE-DERIVED MACROPHAGES [J].

GENG, YJ ;

HANSSON, GK .

JOURNAL OF CLINICAL INVESTIGATION, 1992, 89 (04) :1322-1330

[9]

GERRITY RG, 1981, AM J PATHOL, V103, P181

[10]

Giry C, 1996, J LIPID RES, V37, P1422

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