Electrospinning of collagen type II: A feasibility study

被引:133
作者
Matthews, JA
Boland, ED
Wnek, GE
Simpson, DG
Bowlin, GL
机构
[1] Virginia Commonwealth Univ, Dept Biomed Engn, Richmond, VA 23298 USA
[2] Virginia Commonwealth Univ, Dept Chem Engn, Richmond, VA 23298 USA
[3] Virginia Commonwealth Univ, Dept Anat & Neurobiol, Richmond, VA 23298 USA
关键词
collagen type II; electrospinning; tissue engineering; scaffolding;
D O I
10.1177/0883911503018002003
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Collagen is the natural scaffolding found in all tissues and has been explored extensively for use as a tissue engineering scaffold with limited success. In this feasibility study, the electrospinning of collagen type II and subsequent chondrocyte seeding was investigated for potential use in cartilage tissue engineering. The electrospinning process utilized lyophilized, chicken sternal cartilage collagen type II suspended in 1,1,1,3,3,3 hexaflouro-2-propanol and demonstrated that collagen type II could be electrospun to form nonwoven fibrous mats composed of type II fibers that ranged from 110 nm to 1.8 mum in diameter. The fiber diameter was dependant on the type II concentration in solution with a higher concentration producing the larger diameters. The preliminary chondrocyte seeding study demonstrated that electrospun collagen type II scaffolds support cell growth and are readily infiltrated. In conclusion, the feasibility of collagen type II electrospinning has been demonstrated and the novel scaffolds produced are composed of nano- to micron-scale fiber diameters that have been shown to be compatible with chondrocytes.
引用
收藏
页码:125 / 134
页数:10
相关论文
共 14 条
  • [1] Tailoring tissue engineering scaffolds using electrostatic processing techniques: A study of poly(glycolic acid) electrospinning
    Boland, ED
    Wnek, GE
    Simpson, DG
    Pawlowski, KJ
    Bowlin, GL
    [J]. JOURNAL OF MACROMOLECULAR SCIENCE-PURE AND APPLIED CHEMISTRY, 2001, 38 (12): : 1231 - 1243
  • [2] Bowlin GL, 2002, TISSUE ENGINEERING AND BIODEGRADABLE EQUIVALENTS: SCIENTIFIC AND CLINICAL APPLICATIONS, P165
  • [3] Controlled deposition of electrospun poly(ethylene oxide) fibers
    Deitzel, JM
    Kleinmeyer, JD
    Hirvonen, JK
    Tan, NCB
    [J]. POLYMER, 2001, 42 (19) : 8163 - 8170
  • [4] Freed LE, 1997, IN VITRO CELL DEV-AN, V33, P381
  • [5] CULTIVATION OF CELL-POLYMER CARTILAGE IMPLANTS IN BIOREACTORS
    FREED, LE
    VUNJAKNOVAKOVIC, G
    LANGER, R
    [J]. JOURNAL OF CELLULAR BIOCHEMISTRY, 1993, 51 (03) : 257 - 264
  • [6] Release of tetracycline hydrochloride from electrospun poly(ethylene-co-vinylacetate), poly(lactic acid), and a blend
    Kenawy, ER
    Bowlin, GL
    Mansfield, K
    Layman, J
    Simpson, DG
    Sanders, EH
    Wnek, GE
    [J]. JOURNAL OF CONTROLLED RELEASE, 2002, 81 (1-2) : 57 - 64
  • [7] Mechanical properties of composites using ultrafine electrospun fibers
    Kim, JS
    Reneker, DH
    [J]. POLYMER COMPOSITES, 1999, 20 (01) : 124 - 131
  • [8] BIOCHEMISTRY OF ARTICULAR-CARTILAGE IN HEALTH AND DISEASE
    KUETTNER, KE
    [J]. CLINICAL BIOCHEMISTRY, 1992, 25 (03) : 155 - 163
  • [9] Electrospinning of collagen nanofibers
    Matthews, JA
    Wnek, GE
    Simpson, DG
    Bowlin, GL
    [J]. BIOMACROMOLECULES, 2002, 3 (02) : 232 - 238
  • [10] Mow V. C, 1991, BASIC ORTHOPAEDIC BI, P143